高效液相色谱法测定甜叶菊中甜菊糖总苷的含量.pdfVIP

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高效液相色谱法测定甜叶菊中甜菊糖总苷的含量

关键词：利用高效液相色谱法对甜叶菊干叶中甜菊糖总苷含量开发出一种新的检

测方法，通过单因素试验，确立了检测方法，即样品经30%乙腈超声提取、孔径为

0.45μm的有机滤膜过滤后，选择C18（250mm×4.6mm，5μm）色谱柱；以78%的0.1%

磷酸水溶液+22%乙腈（体积比）为流动相，1.0mL/min的流速进行洗脱。在210nm

波长及40℃柱温下利用DAD检测器检测，用外标法定量。得出的图谱峰形尖锐，结

果回收率高、检测结果准确、精密度高、重复性良好。说明此方法开发具有较强的可

行性。

关键词：高效液相色谱法；甜叶菊；甜菊糖总苷

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Determinationofsteviosideinsteviarebaudianaby

highperformanceliquidchromatography

Absrtact:anewdetectionmethodforsteviosidecontentinsteviarebaudianadry

leaveswasdevelopedbyhighperformanceliquidchromatography.throughsinglefactor

test,thedetectionmethodwasestablished,inotherwords,C(250mm4.6mm,5μm)

×

18

chromatographiccolumnwasselectedafterthesamplewasultrasonicallyextractedwith

30%acetonitrileandfilteredby0.45μmorganicfiltermembrane.Elutionwascarriedout

ataflowrateof1.0mL/minwith78%0.1%phosphoricacidaqueoussolution+22%

acetonitrile(volumeratio)asmobilephase.At210nmwavelengthand40℃column

temperature,DADdetectorwasusedfordetectionandexternalstandardmethodwasused

forquantification.Theobtainedchromatogramhassharppeakshape,highrecoveryrate,

accuratetestresults,pinpointaccuracyandbeautifulrepeatability.Thisshowsthatthe

developmentofthismethodisfeasible.

Keywords:highperformanceliquidchromatography;Steviarebaudiana;Stevioside

totalglycoside