血红蛋白电泳联合地中海贫血基因检测在地中海贫血早期诊断中的应用价值及准确性分析.pdf

CHINESECOMMUNITYDOCTORS论著·临床辅助检查

血红蛋白电泳联合地中海贫血基因检测

在地中海贫血早期诊断中的应用价值及准确性分析

李聪

516001惠州市惠阳区妇幼保健院检验科，广东惠州

doi:10.3969/j.issn.1007-614x.2021.01.046

摘要目的：探索血红蛋白电泳联合地中海贫血基因检测在地中海贫血早期诊断中的应用价值及准确性。方法：选

取健康体检者85例，作为对照组；另选取疑似地中海贫血患者85例，作为观察组。两组均进行地中海贫血基因、血

红蛋白电泳检测，并分析其差异。结果：观察组HbF水平高于对照组，HbA、MCV水平均低于对照组，差异有统计

学意义(P＜0.05)。β-地中海贫血HbF水平高于α-地中海贫血，HbA、MCV水平均低于α-地中海贫血，差异有统

计学意义(P＜0.05)。血红蛋白电泳联合地中海贫血基因诊断正确率为98.65%，灵敏度为98.65%，误诊率为1.35%，特

异度为100.00%，漏诊率为0。结论：在评估地中海贫血患者时，运用血红蛋白电泳联合地中海贫血基因检测，可提

高诊断正确率。

关键词血红蛋白电泳；基因；地中海贫血；诊断；准确性

Applicationvalueandaccuracyanalysisofhemoglobinelectrophoresiscombinedwiththalassemiagenedetectionin

earlydiagnosisofthalassemia

LiCong

DepartmentofLaboratory,theMaternalandChildHealthHospitalofHuiyangDistrict,HuizhouCity,GuangdongHuizhou516001

AbstractObjective:Toexploretheapplicationvalueandaccuracyofhemoglobinelectrophoresiscombinedwiththalassemia

genedetectioninearlydiagnosisofthalassemia.Methods:85healthypeoplewereselectedascontrolgroup.Another85patients

withsuspectedthalassemiawereselectedastheobservationgroup.Thalassemiageneandhemoglobinelectrophoresiswere

performedinbothgroups,andthedifferenceswereanalyzed.Results:Intheobservationgroup,thelevelofHbFwashigherthanthat

ofthecontrolgroup,andthelevelsofHbAandMCVwerelowerthanthoseofthecontrolgroup,thedifferencewasstatistically

significant(P＜0.05).ThelevelofHbFinβ-thalassemiawashigherthanthatinα-thalassemia,andthelevelsofHbAandMCV

werelowerthanthatofα-thalassemia,thedifferencewasstatisticallysignificant(P＜0.05).Thecorrectrateofhemoglobin

electrophoresiscombinedwiththalassemiagenediagnosiswas98.65%,thesensitivitywas98.65%,themisdiagnosisratewas

1.35%,thespecificitywas100.00%,andthemisseddiagnosisratewas0.00%.Conclusion:Intheevaluationofthalassemiapati