针刺对大鼠酒精戒断焦虑症的治疗效果及与CRH的关系.docxVIP

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针刺对大鼠酒精戒断焦虑症的治疗效果及与CRH的关系

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邢宇双郑科文梁启超吴宜艳赵玉佳

[摘要]目的觀察并探究针刺治疗大鼠酒精戒断焦虑症的作用及其机制。方法选取SPF级健康雄性SD大鼠54只，采用完全随机分组方法将其分为空白组、模型（酒精戒断焦虑症模型）组、三阴交组、足三里组、电针两穴（三阴交+足三里）组、针刺两穴（三阴交+足三里）组，每组9只。除空白组外，其余各组均连续腹腔注射酒精28d制备酒精成瘾模型，撤酒精3d制备酒精戒断焦虑症模型，撤酒精期间分别针刺相应穴位进行治疗，针刺3d。采用高架十字迷宫（EPM）测其焦虑行为，酶联免疫吸附测定法（ELISA）检测海马中促肾上腺皮质激素释放激素（CRH）浓度，实时荧光定量PCR法检测海马中CRHmRNA的表达。结果与空白组比较，模型组进入开放臂次数比例和停留时间比例明显减少（P0.05），海马中CRH浓度明显升高（P0.01），CRHmRNA的表达显著升高（P0.01）;与模型组比较，电针两穴组在EPM中进入开放臂次数比例、停留时间比例明显增加（P0.05或P0.01），针刺两穴组进入开放臂次数比例增加（P0.05），电针两穴组和针刺两穴组海马中CRH浓度明显降低（P0.01），CRHmRNA表达亦显著降低（P0.05或P0.01），而单穴位组对各监测指标的作用效果不明显（P0.05）。结论针刺能缓解酒精戒断焦虑症大鼠的焦虑，其抗焦虑的作用可能与调节大鼠海马中CRH的表达有关。

[关键词]针刺;焦虑;酒精戒断;促肾上腺皮质激素释放激素

[]R246.6?????[]A?????[]1673-7210（2019）09（b）-0012-05

EffectofacupunctureforthetreatmentofalcoholwithdrawalanxietyinratsanditsrelationshipwithCRH

XINGYushuang1，2??ZHENGKewen2??LIANGQichao2??WUYiyan2??ZHAOYujia2

1.GraduateSchool，QiqiharMedicalUniversity，HeilongjiangProvince，Qiqihar??161006，China;2.SchoolofPharmacy，MudanjiangMedicalUniversity，HeilongjiangProvince，Mudanjiang??157011，China

[Abstract]ObjectiveToobserveandexploretheeffectandmechanismofacupunctureforthetreatmentofalcoholwithdrawalanxietyinrats.MethodsFifty-fourSPFhealthymaleSDratswereselectedandrandomlydividedintoblankgroup，model（alcoholwithdrawalanxietymodel）group，sānyīnjiāogroup，zúsānlǐgroup，electro-acupuncturetwo-point（sānyīnjiāo+zúsānlǐ）group，acupuncturetwo-point（sānyīnjiāo+zúsānlǐ）group，with9ratsineachgroup.Excepttheblankgroup，alltheothergroupsweregivencontinuousintraperitonealinjectionofalcoholfor28daystopreparethealcoholaddictionmodel，withdrawalofalcoholfor3daystopreparethealcoholwithdrawalanxietymodel，andtheywererespectivelygivenacupunctureatcorrespondingpointsduringwithdrawalofalcoholfor3days.Theelevatedplus-maze（EPM）testwasusedtomeas