雌性大鼠手术去势骨质疏松症模型建立及评价.docxVIP

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雌性大鼠手术去势骨质疏松症模型建立及评价

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陈沙王桂云李荣慧向振吴结枝卓海燕刘平安张国民

〔摘要〕目的从动情周期、骨密度、骨组织形态结构和原代骨髓间充质干细胞（bonemarrowmesenchymalstemcells，BMMSCs）生长曲线4个层次判定雌性大鼠手术去势造模是否成功。方法30只2月龄SPF级雌性大鼠，随机平均分为空白组、假手术组和模型组3组。手术去势造模完成后次日起连续7d对3组大鼠进行阴道脱落细胞涂片，判断动情周期。造模后第8天取左侧股骨组织做骨密度测定和HE染色，取右侧骨组织分离原代BMMSCs。结果空白组和假手术组动情周期正常，模型组大鼠动情周期紊乱;骨组织HE染色显示模型组骨小梁稀少且间距加大;与空白组比较，假手术组骨密度和BMMSCs生长速率差异无统计学意义（P0.05），与假手术组相比，模型组骨密度显著降低，BMMSCs生长速率显著下降，差异具有统计学意义（P

〔关键词〕去势雌性大鼠;骨质疏松症;动物模型;动情周期;骨密度;骨髓间充质干细胞

〔〕R-332???〔文献标志码〕A???〔〕doi：10.3969/j.issn.1674-070X.2020.11.003

〔Abstract〕ObjectiveTodeterminethesuccessoffemaleratssurgicalcastrationmodelfromfourlevelsofestruscycle，bonedensity，bonetissuemorphologyandprimarybonemarrowmesenchymalstemcells（BMMSCs）growthcurve.MethodsThirtytwo-month-oldSPFfemaleratswererandomlyandequallydividedinto3groups：ablankgroup，ashamoperationgroupandamodelgroup.Thevaginalexfoliatedcellsmearswereperformedonthe3groupsofratsfor7consecutivedaysafterthecompletionofthesurgicalcastrationmodeltodeterminetheestruscycle.Onthe8thdayaftermodeling，theleftfemurtissuewastakenforbonedensitydeterminationandHEstaining，andtherightbonetissuewasseparatedfromprimaryBMMSCs.ResultsTheestrouscycleoftheblankgroupandtheshamoperationgroupwasnormal，andtheestruscycleofthemodelgroupwasdisordered.TheHEstainingofbonetissueshowedthatthebonetrabeculaofthemodelgroupwassparseandthespacingincreased;Comparedwiththeblankgroup，thedifferencesinbonedensityandBMMSCsgrowthrateoftheshamoperationgrouphadnostatisticalsignificance（P0.05）.Comparedwiththeshamoperationgroup，thebonedensityofthemodelgroupwassignificantlyreduced，andthegrowthrateofBMMSCswassignificantlyreduced.Thedifferencewasstatisticallysignificant（P

〔Keywords〕ovariectomizedfemalerat;osteoporosis;animalmodel;estrouscycle;bonedensity;bone