基于网络药理学的延胡索干预心力衰竭作用机制研究.docxVIP

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基于网络药理学的延胡索干预心力衰竭作用机制研究

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陈晶王萌侯志涛

摘要：目的??采用网络药理学方法探究延胡索干预心力衰竭可能的作用机制。方法??检索中药系统药理学数据库与分析平台（TCMSP）、中国天然产物化学成分库，根据口服生物利用度（OB）≥30%、类药性（DL）≥0.18、生物半衰期（HL）≥4.00对延胡索可能的生物活性成分进行筛选，并通过蛋白质数据库Uniprot筛选出各活性成分可能存在的靶点，通过CTD、TTD、DrugBank数据库挖掘心力衰竭相关的作用靶点。通过基因注释工具Metascape分析延胡索干预心力衰竭的分子机制。结果??筛选出延胡索生物活性成分26个，预测出延胡索与心力衰竭相关的靶点210个，蛋白质相互作用网络构建筛选出关键靶点37个。Metascape分析顯示，延胡索干预心力衰竭的关键靶点主要与炎症反应、细胞凋亡和调节肠道菌群等生物过程有关。结论??延胡索不仅可直接对心力衰竭起到治疗作用，还可通过抗炎、抑制细胞凋亡及调节肠道菌群间接发挥疗效。

关键词：延胡索;心力衰竭;网络药理学;信号通路;作用机制;肠道菌群

：R259.416.1;R285.5????：A????：1005-5304（2020）03-0071-07

DOI：10.3969/j.issn.1005-5304.201903319

Study?on?Mechanism?of?Intervention?of?Corydalis?Rhizoma?in?Heart?Failure?Based?on?Network?Pharmacology

CHEN?Jing，?WANG?Meng，?HOU?Zhitao

Heilongjiang?University?of?Chinese?Medicine，?Harbin?150040，?China

Abstract：?Objective?To?explore?the?possible?mechanism?of?intervention?of?Corydalis?Rhizoma?in?heart?failure?by?using?network?pharmacology.?Methods?TCMSP?and?chemical?separation?of?natural?products?in?China?were?used.?According?to?oral?bioavailability?（OB）?≥30%，?drug-likeness?（DL）?≥0.18，?biological?half-life?（HL）?≥4.00，?bioactive?components?of?Corydalis?Rhizoma?were?screened，?and?the?possible?targets?of?each?active?components?were?screened?out?through?the?protein?database?Uniprot.?Heart?failure-related?targets?were?explored?through?the?CTD，?TTD，?and?DrugBank?databases.?The?molecular?mechanism?of?intervention?of?Corydalis?Rhizoma?in?heart?failure?was?analyzed?by?the?gene?annotation?tool?Metascape.?Results?Totally?26?bioactive?components?of?Corydalis?Rhizoma?were?screened?out，?and?210?targets?of?interventing?heart?failure?were?predicted.?The?PPI?network?was?constructed?to?screen?37?key?targets.?Metascape?analysis?showed?that?the?key?targets?of?Corydalis?Rhizoma?intervention?in?heart?failure?were?mainly?related?to?inflammatory?response，?apoptosis?and?regulation?of?intestinal?flora?and?other?biological?processes.?Conclusion?Corydalis?Rhizoma?can?not?only?directly?treat?heart?failure，?but?also?indirec