黄芩苷对白色念珠菌酶活性的影响.docx

黄芩苷对白色念珠菌酶活性的影响

【摘要】目的运用分光光度法检测黄芩苷作用白色念珠菌后，其琥珀酸脱氢酶(SDH)、细胞色素C氧化酶(CC0)、Ca2+-Mg2+-ATPase活性的改变，以探讨黄芩苷抗白色念珠菌作用靶位，为探索中药活性成分抗真菌作用机理提供依据。方法收集经黄芩苷作用了相应时间的白色念珠菌，制备菌悬液，反复冻融研磨，差速离心法提取白色念珠菌线粒体；Lowry法检测线粒体蛋白质含量；分光光度法检测SDH酶、CCO酶、Ca2+-Mg2+-ATPase活力。结果①0.25,0.5,1mg/ml黄芩苷浓度组白色念珠菌SDH活力明显低于0mg/ml黄芩苷浓度组(P0.05),且出现黄芩苷浓度越高SDH活力越低的趋势。不同作用时间组间白色念珠菌SDH活力差异无统计学意义(P0.05)。②白色念珠菌经不同浓度黄芩苷作用不同时间后，其CCO酶活力均无明显差异(P

0.05)。③0.25,0.5,1mg/ml黄芩苷浓度组与0mg/ml黄芩苷浓度

组比较，Ca2+-Mg2+-ATPase活力差异显著,黄芩苷浓度越高，Ca2+-Mg2+-ATPase活力越低，作用时间对Ca2+-Mg2+-ATPase活力没有明显影响(P0.05)。结论①黄芩苷具有明显的抗白色念珠菌作用；②黄芩苷可降低白色念珠菌SDH酶活力；③黄芩苷对白色念珠菌CCO酶活力未见明显影响；④黄芩苷可降低白色念珠菌Ca2+-Mg2+

-ATPas

【关键词】黄芩苷白色念珠菌抗真菌

Abstract:0bjectiveToinvestigatethetargetsiteofbaicalinagainstCandidaalbicansandsupplybaseforexploringantifungalactionmechanismofthetraditionalChinesemedicineactivecomponentbydetectingtheeffectofbaicalinonsuccinatedehydrogenase,cytochrome-oxydase,Ca2+-Mg2+-ATPasethroughabsorptionspectrometry.MethodsAfterincubatedwithdifferentconcentrationsofbaicalinforcorrespondingtime,Candidaalbicanswasharvestedandresuspendedinphysiologicalsaline,whichwasfollowedbyfreezethawingandgrindthreetimes.Thenmitochordriawasextractedbydifferentcentrifugationandusedassample.Lowrymethodwasappliedtodetecttheproteincontentandabsorptionspectrometrywasused

todetecttheactivitiesofSDH,CCO,Ca2+-Mg2+-ATPase.ResultsItwasshowedthat①theactivityofSDHofCandidaalbicansincontrolgroupwasmuchhigherthanthatofCandidaalbicans

treatedwithbaicalinatthreedosesof0.25

mg/ml,0.5mg/ml,1mg/ml,whilenodifferencewasobservedamongthethreedoses.②NodifferencewasobservedintheactivityofCCOofCandidaalbicanstreatedwithdifferentdosesofbaicalin(Pgt;0.05)fordiff