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USEOFBIOREACTORSINAUTOLOGOUSCHONDROCYTECULTUREOVERTHREEDIMENSIONALBIODEGRADABLESCAFFOLDSTOREPAIRARTIFICIALLYCREATEDCHONDRALDEFECTSINRABBIT.By:Dr.KumarPriteshDeptt.OfOrthopaedicsGuide:-Prof.A.RastogiDepttofOrthopaedicsIMSBHUCo–Guides:Dr.PradeepSrivastavaSchoolofBiochemicalEngineering,IITBHUDrAmritaGhoshkarDeptt.oFPathologyIMSBHU
AHOPELESSSITUATIONINDEED
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RECONSTRUCT?
AIMSANDOBJECTIVES:
WHYACCPRODUCTIONON3DSCAFFOLDS??Articularcartilagedefect–amajorhealthproblemduetolimitedcapacityofself-regeneration.Leadstopainandosteoarthritis.Traumacausinginjurytosubchondralbone–fibrocartilagetissue–badcartilage.Partialthicknessinjury–avascularhealing–poorTissuecultureofcartilageiscostly.Culturedchondrocyteshavetendencytode-differentiate.Transplantedcellscanleakintojoint,sizeofdefectislimited,requiresintactadjacentcartilage.
HYPOTHESIS:Thesolution3-Dbiodegradablescaffoldsprovidethenecessarymatrixstructureforchondrocytes.bio-reactorproducesbetteryieldandqualityofcartilagecellsbysimulatingstressandstrainconditionsofjoint.impregnatedchondrocyteshavenochanceofleakagedonotrequireopentechniqueasnosuturesinvolved.bio-degradablescaffoldsdonotactasforeignmaterialmaterialischeap–samewhichweuseinabsorbablesutures.futureadvancementwithtopographicalimagingcanhelpusbuildcustomised3Dreplicaofthe“missing”pieceandtheyarethematerialofchoicefortheimplant.
METHODOLOGYDefectsof2.5mmdiameternotextendingtothezoneofcalcificationwillbecreatedinarticularcartilageofbothkneeofeachrabbitinthenonweightbearinginter-condylarregion.
METHODOLOGYBitsofcartilagethusobtainedwillbeculturedafterisolationandinitialprocessingoverthreedimensionalbiodegradablescaffoldsundercontrolledbiophysicalconditionsinabioreactorfor2wks
METHODOLOGYBitsofcartilagethusobt
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