DB46T 646-2024《斑兰叶（香露兜）组培快繁技术规程》.docxVIP

ICS65.020.20

4

6

CCSB61

海南省地方标准

DB46/T646—2024

斑兰叶（香露兜）组培快繁技术规程

Technicalcodeofpracticefortissuecultureandrapidpropagationofpandan

2

024-09-23发布

2024-11-01实施

海南省市场监督管理局发布

DB46/T646—2024

目次

前言................................................................................III

引言.................................................................................IV

1

2

3

4

5

范围...............................................................................1

规范性引用文件.....................................................................1

术语和定义.........................................................................1

组培工厂要求.......................................................................1

接种前准备.........................................................................2

5

5

5

.1试剂...........................................................................2

.2灭菌...........................................................................2

.3人员要求.......................................................................2

6

7

外植体采集与处理...................................................................2

6

6

6

.1采集圃选择.....................................................................2

.2外植体采集.....................................................................2

.3外植体处理.....................................................................3

组培快繁...........................................................................3

7

7

7

7

7

7

7

.1接种与检查.....................................................................3

.2愈伤组织诱导...................................................................3

.3丛生芽诱导.....................................................................3

.4增殖培养.......................................................................3

.5丛生芽循环再生.................................................................3

.6生根培养.......................................................................3

.7炼苗..........