血管内皮生长因子与细胞支架复合物修复大鼠股骨缺损.pdfVIP

血管内皮生长因子与细胞支架复合物修复大

鼠股骨缺损

徐成振,马鹏,徐晓峰,李阳,钱栋

【摘要】目的：探讨血管内皮生长因子(VEGF)与骨髓间充质干

细胞（MSCs）、纳米晶胶原基骨(nHAC)复合物对大鼠股骨缺损的修复作

用。方式：将20只SD大鼠制成股骨缺损模型后分2组：对照组植入

MSCs/nHAC复合物，实验组植入VEGF/MSCs/nHAC复合物。术后第2，4，

8周行影像学和组织学观看;术后第8周行新生骨痂环境扫描电镜

（ESEM）检查。结果：术后第2，4，8周实验组与对照组放射学检查

评判骨生成不同有统计学意义(Plt;。组织学观看发觉实验组较对照

组能更快更有效地增进大鼠股骨缺损处的骨痂生长。结论：

VEGF/MSCs/nHAC支架较MSCs/nHAC支架对骨缺损的修复有更好的成

效。

【关键词】血管内皮生长因子；骨髓间充质干细胞；纳米晶胶

原基骨；骨缺损；环境扫描电镜

［Abstract］Objective:Toevaluatetheeffectofvascular

endothelialgrowthfactor（VEGF）combinedwiththecompoundof

marrowmesenchymalstemcells(MSCs)andnano

hydroxyapatite/collagen(nHAC)boneonthetreatmentofrat

femoralbone：Animalmodelsoffemoralbonedefectwere

establishedin20SpragueDawleyweredividedinto2groups

randomly:Incontrolgroup，defectwasfilledwithnHAC/MSCs

scaffolds;inexperimentgroupwasfilledwithMSCs/VEGF/and

histologicalobservationweremadeinthe2nd，4thand8thweeks

afterscanningelectronmicroscope(ESEM)observationwasmade

inthe8thweekafteroperation.Results：Thereweresignificant

differencesinboneregenerationbetweencontrolgroupand

experimentgroupinthe2nd，4thand8thweeksafter

operation(Plt;.Histomorphologyobservationshowedthatnew

osteotylusofexperimentgroupwasearlierandmorethancontrol

group.Conclusion：Thebonedefectrepairabilityoftissue

engineeringbonewhichconstructedwithVEGF/MSCs/nHACwas

strongerthanMSCs/nHAC.

［Keywords］vascularendothelialgrowthfactor;marrow

mesenchymalstemcells;nanohydroxyapatite/collagen;bone

defect;environmentscanningelectronmicrosc