过度表达通过直接靶向抑制神胶质瘤细胞恶性肿瘤r20 mir032stcc krasmir.pdfVIP

Neuro-Oncology15(11):1491–1501,2013.

doi:10.1093/neuonc/not107NEURO-ONCOLOGY

AdvanceAccesspublicationOctober3,2013

Overexpressedlet-7ainhibitsgliomacell

malignancybydirectlytargetingK-ras,

tlyofPTEN

††††

Xi-RuiWang,HuiLuo,Hai-LinLi,LeiCao,Xie-FengWang,WeiYan,Ying-YiWang,

Jun-XiaZhang,TaoJiang,Chun-ShengKang,NingLiu,andYong-PingYou,

GliomaCooperativeGroup(CGCG)

DepartmentofNeurosurgery,TheFirstAffiliatedHospitalofNanjingMedicalUniversity,Nanjing,

(X.-R.W.,H.L.,H.-L.L.,L.C.,X.-F.W.,Y.-Y.W.,N.L.,Y.-P.Y.);DepartmentofNeurosurgery,TianjinMedical

UniversityGeneralHospital,Tianjin,(J.-X.Z.,C.-S.K.);BeijingNeurosurgicalInstitute,Departmentof

Neurosurgery,BeijingTiantanHospital,CapitalMedicalUniversity,Beijing,(W.Y.,T.J.);ThirdDepartment

ofNeurosurgery,CangzhouCentralHospital,Cangzhou,(X.-R.W.)

Background.Alteredexpressionofmicro(mi)RNAshasinducesuppressionofgliomainvivobygeneratinga

beenshowntobeassociatedwithtumorigenesisandgliomaxenograftmodel.

tumorprogression.TheexpressionofphosphataseandConclusion.Ourresultsindicatedthatlet-7asuppresses

tensinhomolog(PTEN)playsanimportantroleinitstargettranscriptK-rasandinhibitsgliomamalignancy

gliomaandisregardedasaprognosticmarkerofgliomatofPTENexpression.

patients.Thegoalofthisstudywastoinvestigatethe

functionoflethal(let)-7amiRNAingliomacelllinesKeywords:glioma,let-7a,K-ras,miRNA,PTEN.

withdifferentPTENphenotypes.

Methods.Onehundredninety-eightgliomatissueswere