甲基化靶向测序 流程.pdfVIP

甲基化靶向测序流程

英文回答：

MethylationTargetedSequencingWorkflow.

Methylationtargetedsequencingisapowerfultechnique

usedtoidentifyandquantifyDNAmethylationpatternsat

specificgenomicregions.Thisinformationcanprovide

valuableinsightsintoepigeneticregulation,disease

development,andtherapeuticresponse.Theworkflowfor

methylationtargetedsequencinginvolvesseveralkeysteps:

1.DNAExtractionandLibraryPreparation:GenomicDNA

isextractedfromthesampleofinterest.DNAlibrary

preparationinvolvesfragmentingtheDNA,ligatingadapters,

andamplifyingthefragmentstocreatealibrarysuitable

forsequencing.

2.MethylationEnrichment:Variousmethodscanbeused

toenrichformethylatedorunmethylatedDNAfragments.

Bisulfiteconversionisacommontechniquethatconverts

unmethylatedcytosinestouracils,whilemethylated

cytosinesremainunchanged.Thisallowsfortheselective

amplificationandsequencingofmethylatedregions.

3.Sequencing:Theenrichedlibraryissequencedusing

next-generationsequencing(NGS)platformstoobtainhigh-

throughputdataonthemethylationstatusofthetargeted

regions.

4.DataAnalysis:Thesequencingdataisanalyzedto

identifyandquantifythemethylatedandunmethylated

regions.Thisinvolvesmappingthereadstothereference

genome,identifyingmethylation-specificpatterns,and

performingstatisticalanalysistodeterminesignificant

methylationdifferences.

5.Interpretation:Themethylationpatternsare

interpretedtounderstandtheirbiologicalsignificance.

Thiscanincludeidentifyingdifferentiallymethylated

regions(DMRs)associatedwithspecificdiseasesor

conditions,correlatingmethylationpatternswithgene

expression,anddevelopingepigeneticbiomarkersfor

diagnosisorprognosis.

中文回答：

甲基化靶向测序流程。

甲基化靶向测序是一种用于识别和量化特定基因组区域中DNA

甲基化模式的有力技术。此信息可以为表观遗传调控、疾病发生和

治疗反应提供有价值的见解。