数据警报-光度测定基础技术培训任务data alarms photometry v2.pptx

数据警报-光度测定基础技术培训任务data alarms photometry v2.pptx

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DataAlarms-Photometric

BasicTechnologyTraining

BTTDataAlarmsPhotometricv.2Task:Collectallpiecesofthealarmdescription.(6pages)Prepareshortpresentation:whatdoesthealarmmeanwhatcanbedonetogetcorrectresult

BTTDataAlarmsPhotometricv.2

BTTDataAlarmsPhotometricv.2ExcessiveAbsorbance ABS?or“ABS” ZTheabsorbancevalueusedforcalculation(aftercellblankcorrection)exceeds3.3Abs.Abslimit3.3

BTTDataAlarmsPhotometricv.2ABS?or“ABS”Areallsamplesaffectedoronlyone?Ifonlyone Checksample-lipaemicIfall Checkreagentpreparation Checkopticalpathofphotometer Checkqualityofreactioncell(intermittentalarm)

BTTDataAlarmsPhotometricv.2allpointsover LIMT0 I

exc.of1point LIMT1 J

2or3points LIMT2 KUtility-Application-AnalyzeAbsorbancelimitOnc6000ReactValidforRateassaysonly(including2pointRateassay)

BTTDataAlarmsPhotometricv.2Absorbancelimit-Kineticmeasuringrange

BTTDataAlarmsPhotometricv.2Absorbancelimit-Kineticmeasuringrange

BTTDataAlarmsPhotometricv.2?SampleAbs.–Std1Absismeasured.?AbsorbancelimitvalueisadjustedaccordinglywhencheckingforsubstratedepletionAbsorbancelimit

BTTDataAlarmsPhotometricv.2Whatissubstratedepletion?C+DA+BEnzymeXAandBarethesubstrates–thesubstanceswhichareconvertedbytheenzymeThereactioncancontinueuntiloneorbothofthesubstratesisallusedup(exhausted).Thismaytakeseconds,minutes,hoursordaysdependingonhowfastthereactionis.Themoreenzymepresent?thefasterthereaction

BTTDataAlarmsPhotometricv.2Noamountofwaiting(time)willproducemorecouples–nosubstratetoconvert!Whatissubstratedepletion?No.ofweeksNo.ofcoupleomoremen

BTTDataAlarmsPhotometricv.2 Themainwavelengthabsorbanceexceededthereactionlimitatthe3rdor4thandsubsequentphotometricpointsusedforcalculation.Themoreenzymepresent?thefasterthereactio

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