沉默MDR1基因增强SGC7901_ADM细胞对姜黄素的敏感性_孙新迪.pdfVIP

基因组学与应用生物学，2017年，

第36卷，第6期，第2486-2490页

GenomicsandAppliedBiology,2017,Vol.36,No.6,2486-2490

研究报告

ResearchReport

沉默MDR1基因增强SGC7901/ADM细胞对姜黄素的敏感性

孙新迪邵淑丽*恽冬泽李旭艳张伟伟付博李妍

齐齐哈尔大学生命科学与农林学院,齐齐哈尔,161006

*通讯作者,shshl32@163.com

摘要本研究利用短发夹RNA(shRNA)沉默SGC7901/ADM细胞MDR1基因表达，增强人胃癌

SGC7901/ADM细胞对姜黄素的敏感性。根据MDR1基因序列设计3对编码shRNA的DNA模板，克隆到

pSilencer3.1-H1neo(p3.1)上构建3种shRNA表达载体，转染SGC7901/ADM细胞，qRT-PCR和Western

blotting检测MDR1基因沉默效果，用荧光显微镜观察细胞形态，MTT法检测细胞活力。结果显示，3种

shRNA表达载体转染细胞后均能不同程度沉默MDR1基因的表达，增强了细胞对姜黄素的敏感性。

关键词SGC7901/ADM细胞,MDR1,shRNA,姜黄素

SilencingMDR1GeneEnhancestheSensibilityofSGC7901/ADMCellsto

Curcumin

SunXindiShaoShuli*YunDongzeLiXuyanZhangWeiweiFuBoLiYan

CollegeofLifeSciences,AgricultureandForestry,QiqiharUniversity,Qiqihar,161006

*Correspondingauthor,shshl32@163.com

DOI:10.13417/j.gab.036.002486

AbstractThisdissertationresearchessomequestionsaboutusingshorthairpinRNA(shRNA)tosilence

MDR1geneexpressionofcellsSGC7901/ADMtoenhancethesensibilityofhumangastriccancercells

SGC7901/ADMtowardscurcumin.AccordingtoMDR1genesequence,threepairsofDNAtemplatewhich

encodingshRNAhadbeendesigned,andclonethemtopSilencer3.1-H1neo(p3.1)toconstructthreekindsof

shRNAexpressionvectors.Afterthat,theSGC7901/ADMweretransfected,andWesternblottingwasappliedto

detectthesilenceeffectsofMDR1gene,fluorescencemicroscopewasusedtoobservecellmorphologyandMTT

methodwasadoptedtodetecttheviabilityofcellsSGC7901/ADM.Theresultsshowedthatcells,transfectedby

thesethreeshRNAexpressionvector,couldsilencetheexpressionofMDR1geneindifferentdegrees,andcould

enhancethesensibilitycellsofSGC7901/ADMtowardscurcumin.

KeywordsSGC7901/ADMcell,MDR1,shRNA,Curcumin

胃癌是常见的癌症之一，严重威胁着人类的身体glycoprotei