莪术油诱导人胃腺癌SGC_7901细胞凋亡的研究_李玲玲.pdfVIP

ChineseJournalofCellBiology2015,37(9):1235–1241DOI:10.11844/cjcb.2015.09.0173

SGC-7901

(,161006)

(zedorayturmericoil,ZTO)SGC-7901

ZTOSGC-790148h,

;;

DNA;AnnexinV-FITC;PCRWesternblot

BaxBcl-2mRNA,SGC-790148h,ZTO

110μg/mL,IC50(108.002±0.305)μg/mL;;DNA

;48h(25.07±0.82)%;Bax,Bcl-2

,Bax/Bcl-2(P0.05),ZTOBaxBcl-2

SGC-7901

;SGC-7901;

ResearchofZedoaryTurmericOilInducedApoptosisofHumanGastric

CarcinomaSGC-7901Cells

LiLingling,ShaoShuli*,SunHongyan,ZhangWeiwei

(CollegeofLifeSciencesandAgricultureandForestry,QiqiharUniversity,Qiqihar161006,China)

AbstractThestudyofthisexperimentwastoexploretheeffectsfortheapoptosistriggedbyzedoary

turmericoil(ZTO)onthehumangastriccarcinomaSGC-7901cells.TheeffectsofZTOwerestudiedbytrypan

bluestainingmethod,lightmicroscope,fluorescencemicroscope,transmissionelectronmicroscope,flowcytometry,

Real-timePCRandWesternblot.AfterthehumangastriccarcinomaSGC-7901cellstreatedwithvarious

concentrationsofZTOfor48h,thegrowthinhibitionrateofcellwasdeterminedbytrypanbluestainingmethod.

Themorphologyandultrastructurewereobservedbylightmicroscope,fluorescencemicroscopeandtransmission

electronmicroscope.DNAfragmentationwasassayedbyagarosegelelectrophoresis.Theapoptoticratewas

investigatedbyflowcytometry.TheexpressionlevelsofBaxandBcl-2wereexploredbyReal-timePCRand

Westernblot.Theresultsshowedthattheconcentrationforthetreatmentwasoptimizedto110μmol/Linthisstudy,

andtheIC50valuewas(108.002±0.305)μg/mL.Theearlyapoptoticratewasreachedto(25.07±0.82