适配骨组织工程的蚕丝增强聚己内酯复合材料理化及生物特征研究.docxVIP

适配骨组织工程的蚕丝增强聚己内酯复合材料理化及生物特征研究

目录

一、内容概要................................................3

1.研究背景..............................................3

2.研究意义..............................................4

3.研究目的与主要研究内容................................5

二、材料制备与表征..........................................6

1.蚕丝的预处理与改性....................................7

蚕丝的脱胶.............................................8

蚕丝的漂白.............................................9

蚕丝的稳定化处理......................................10

2.聚己内酯的合成与纯化.................................11

3.复合材料的制备.......................................11

溶液共混法............................................12

熔融共混法............................................13

4.材料表征.............................................14

三、理化性能测试...........................................16

1.力学性能测试.........................................17

压缩强度..............................................18

拉伸强度..............................................19

冲击强度..............................................20

2.热性能测试...........................................21

热变形温度............................................21

热导率................................................22

3.化学稳定性测试.......................................23

盐雾试验..............................................24

酸碱腐蚀实验..........................................25

四、生物相容性与细胞毒性评价...............................26

1.生物相容性测试.......................................27

细胞毒性实验..........................................28

皮肤刺激实验..........................................29

血液相容性测试........................................30

2.组织工程应用实验.....................................31

骨缺损修复实验........................................31

软组织修复实验........................................32

五、动物实验与临床前研究...................................33

1.动物实验.............................................34

实验动物选择..........................................3