肺痿论治干预肺纤维化大鼠肺功能的实验研究.pdfVIP

肺痿论治干预肺纤维化大鼠肺功能的实验研究

康荻;李亚东;李彧;朱曼;唐磊;申萌萌;常立国;黄春芳;王继峰;牛建昭

【摘要】目的:比较麦门冬汤和甘草干姜汤在肺纤维化阶段对大鼠模型肺功能的干

预作用.方法:用博莱霉素造模14d的SD大鼠,灌胃麦门冬汤(7.5g/kg、15g/kg、

30g/kg)和甘草干姜汤组(3.75g/kg、7.5g/kg、15g/kg),模型组和正常组大鼠

灌胃等体积饮用水.中药灌胃14d后检测用力肺活量(FVC)、肺动态顺应性(Cdyn)、

0.4秒率(FEV0.4/FVC%)、呼气峰值流速(PEF)、最大呼气中期流速(MMF)等指标,

检测血气变化,称量脏器质量、计算脏器系数,HE染色观察肺组织病理.结果:麦门冬

汤高剂量组能够降低模型动物肺脏质量、肺脏系数(P0.05),提高肺顺应性、FVC

和质量FVC(P0.05);甘草干姜汤高剂量能够改善肺顺应性和FVC(P0.05);甘草干

姜汤中剂量能够改善质量FVC、降低0.4秒率(P0.05),保护非纤维化区肺泡结构;

甘草干姜汤能够提高肺纤维化大鼠的PO2和SO2(P0.05).结论:麦门冬汤和甘草

干姜汤对博莱霉素所致肺纤维化大鼠肺脏功能均具有改善作用,前者主要是改善气

道周围纤维化区的间质增生,后者主要是保护非纤维化区的肺泡结构和功

能.%Objective:ToobservedifferenteffectsofMaimendongDecotionand

GancaoGanjiangDecotioninonpulmonaryfibrosisratinthestageof

fibroticphase.Methods:After14daysofestablishingratmodelby

intratrachealinjectionofbleomycin,Maimendongdecotion(7.5g/kg,15

g/kg,30g/kg)andGancaoGanjiangdecotion(3.75g/kg,7.5g/kg,15g/kg)

weregivenbygavageonceadayfor2week.Meanwhile,themodelgroup

andthenormalgroupwereadministeredwithdistilledwaterofsame

quantity.Aftergavageofdecotionfor14days,theforcedvitalcapacity

(FVC),theindexesoflungdynamiccompliance(Cdyn),theratioofFEV0.4to

forcedvitalcapacity(FEV0.4/FVC%),peakexpiratoryflowrate(PEF),maximal

mid-expiratoryflowrate(MMF)andthebloodgaschangeswere

recorded,thentheorgancoefficientswerecalculated,lungtissueand

airwaypathologicchangeswereobservedbyHE

staining.Results:Comparedwiththemodelgroup,thehighdosegroupof

Maimendongdecotionreducedthelungweightandlungindex

significantly(P0.05),andincreasedCdyn,FVCandFVC/lungweight

significantly(P0.05).TheCdynandFVCofhighdosegroupofGancao

Ganjiangdecotionwerehigherthanthemodelgroup(P0.05).The

intermediate-doseGancaoGanjiangdecotionimprovedFVC/lungweight

(P0.05)andreducedtherateofFEV0.4/FVC%significantly(P0.05)

comparedwiththem