基于网络药理学方法研究补肾活血方治疗肾纤维化药效物质基础及分子作用.pdf

基于网络药理学方法研究补肾活血方治疗肾纤维化药效物质基

础及分子作用机制

杜霞;李鹤鸣;陈志永;郭冬;王春柳;李晔

【摘要】目的:探讨补肾活血方治疗肾纤维化的药效物质基础及分子作用机制.方法:

基于网络药理学理论框架,结合ADME性质筛选、靶点预测、网络构建及KEGG

通路富集分析等方法进行研究.结果:补肾活血方中5味药材共包含491个化合物,

其中具有良好的ADME性质的188个潜在活性成分中,共有136个活性成分及其

对应的59个靶标与补肾活血方治疗肾纤维化密切相关.通过构建\药材-活性成分-

靶标\网络及对网络进行分析,共获得19个关键化合物和5个关键靶标.此外,在对

靶标进行KEGG通路分析时,共获得19条与补肾活血方治疗肾纤维化相关的通路,

其中,PI3K-Akt信号通路、MAPK信号通路、HIF-1信号通路为主要通路.结论:补

肾活血方可通过多成分、多靶标及多通路的作用模式治疗肾纤维化.本研究为补肾

活血方治疗肾纤维化的药效物质及潜在作用机制研究提供了一种新的研究思路,为

该方剂的进一步升级改造及临床研究提供了必要的理论依

据.%Objective:Investigatethepharmacodynamicmaterialbasisand

molecularmechanismofBushenHuoxueprescriptionintreatmentofrenal

fibrosis.Methods:Basedonthetheoreticalframeworkofnetworkphar-

macology,ADMEscreening,targetprediction,networkconstructionand

KEGGpathwayenrichmentanalysiswereappliedforthis

work.Results:Therewere491compoundsinthe5medicinalherbsof

BushenHuoxueprescription.Inthesecompounds,188compoundswere

aspotentialactivecomponentswithgoodADMEproperties,and136ac-

tivecompoundsandcorresponding59targetswerecloselyrelatedto

BushenHuoxueprescriptioninthetreatmentofrenalfibrosis.By

constructingandanalyzingtheactiveingredients-targetsnetwork,19key

compoundsand5keytargetswereobtained.Inaddition,theKEGG

pathwayanalysisshowedthat19pathwaysrelatedtotheBushenHuoxue

prescriptionforkidneyfibrosis,andPI3K-Aktpathway,MAPKsignaling

pathwayandHIF-1signalingpathwaywerethemajor

pathways.Conclusion:BushenHuoxueprescriptioncantreatrenalfibrosis

throughmulti-component,multi-targetandmulti-pathway.Thisstudy

providesanewideaforthestudyofthepharmacodynamicsubstancesand

potentialmechanismofBushenHuoxueprescriptioninthetreatmentof

renalfibrosis,andprovidesthenecessarytheoreticalbasisforthefurther

upgradingandclinicalresearchof