γ-干扰素对金黄色葡萄球菌性临床型乳房炎的疗效研究.pdfVIP

γ-干扰素对金黄色葡萄球菌性临床型乳房炎的疗效研究

马定坤;伍杰;刘娟;程跃红

【摘要】本试验选取32只3～4kg健康泌乳家兔,通过人工造模的方法诱发其乳

腺发炎,建立金黄色葡萄球菌性临床型乳腺炎模型.然后随机分为A(γ-干扰素)、

B(乳酸环丙沙星)、C(γ-干扰素和乳酸环丙沙星)、D(生理盐水)4个治疗组,每组8

只,于造模后6、18h各投药一次.各组分别在造模前lh和造模后4、12、24、36、

48、72h采集血液0.5mL、血清1mL,进行白细胞总数、血清钙离子含量和炎性

细胞因子测定(ELISA),研究其治疗效果.结果显示:①外周血白细胞总数组间对比,A、

B组在造模后12～48h内极显著高于C组(P＜0.01),72h时显著高于C组(P＜

0.05),但两组间无显著差异(P＞0.05);D组在造模后12～72h内均极显著高于其他

3组(P＜0.01).②血清钙离子含量组间对比,A、B组在造模后12～48h内极显著低

于C组(P＜0.01),72h时显著低于C组(P＜0.05),但两组间差异不显著(P＞0.05)；

D组在造模后12～72h内均极显著低于其他3组(P＜0.01).③血清中TNF-α和

IL-6含量组间对比,A、B组在与C组造模后12～24h内差异显著(P＜0.05),36～

48h内差异极显著(P＜0.01),72h后差异显著(P＜0.05),A、B两组间无显著差异

(P＞0.05)；D组在造模后12～72h内均极显著高于其他3组(P＜0.01).结果表

明,γ-干扰素配合乳酸环丙沙星治疗金黄色葡萄球菌性临床型乳房炎具有一定的协

同作用.%Intheexperiment,104CFU/mL/3to4kg(bodyweight)of

Staphylococcusaureusthatwasisolatedfromthemilkofdairycattlewith

mastitiswereinfusedintothethirdofmammaryglandin32healthy

rabbits,thentheclinicalmastitismodelwerechallenged.32rabbitswere

randomlydividedintoA(IFN-y),B(Ciprofloxacin),C(IFN-yand

CiprofloxacinLac-tate),D(normalsaline)asfourtreatmentgroups,each

contains8rabbits,thendeliverydrugonlyafter6,18hasfirstdosage.Each

group,respectively,frombeforeestablishedmodel1,4,12,24,36,48,72h

observetheirtemperature,heartrateandrespiratoryrateandotherclinical

indicators,andcollectblood0.5mL,serum1mL.forbloodandblood

biochemicaldetection,anddeterminationofinflammatorycytokines

(ELISA),tostudyitstherapeuticeffect.Theresultsshowedthat:①12to48

hafterchallenged,A,Bgroup,thetotalnumberofperipheralwhiteblood

cellswassignificantlyhigherthantheCgroups(P0.01),andwashigher

thantheCgroup(P0.05)whenthe72hafterchallenged,butinter-

groupnosignificantdifference(P0.05);12to72hafterchallenged,D

groupwassignificantlyhigherthantheA,B,Cgr