经股动脉注射途径建立前列腺癌和乳腺癌骨转移模型 .pdfVIP

经股动脉注射途径建立前列腺癌和乳腺癌骨转移模型

殷昭阳;齐思勇;成祥;郭靓;陈泓宇;施明

【摘要】ObjectiveToexploreanefficientandreliablemethodfor

establishingananimalmodelofbonemetastasisoftumors.MethodsMale

C57BL/6Jmicewererandomizedinto4equalgroupstoreceiveinjections

ofnormalsalineorprostatecancerRM-1cellsuspensionviathefemoral

arteryortheexternaliliacartery,andbreastcancer4T1-luccellswere

injectedin15femaleBALB/cmiceviathefemoralartery.Theoperation

time,postoperativesurvivalrate,andthetumorformationratesinthe

micewithtwodifferentinjectionmethodswerecompared.Thetumor

metastasisinthemicewasevaluatedwithinvivoimaging.ResultsThe

meantimeforhemostasistimewas2.53±1.75mininmicereceiving

tumorcellinjectionviathefemoralartery,significantlyshorterthanthatin

micewithinjectionsviatheexternaliliacartery(4.70±1.63min;P0.05);

themeanoperationtimewas14.67±2.16minand22.47±3.50mininthe

twogroups,respectively(P0.05).At21daysaftertheoperation,the

survivalratewas93.3%infemoralarteryinjectiongroup,significantly

higherthanthatinexternaliliacarteryinjectiongroup(66.7%;P0.05).The

tumormetastasisratewas100%inbothgroups.ConclusionInjectionof

thetumorcellsviathefemoralarteryismoresuitableforestablishing

mousemodelsofbonemetastasisofcancers.%目的探索建立高效、可靠的肿

瘤骨转移动物模型的方法.方法将C57BL/6J雄鼠随机分为4组,每组15

只;BALB/c雌鼠15只,设一组.将小鼠前列腺癌细胞RM-1和乳腺癌细胞4T1-luc

消化、洗涤、计数后用生理盐水重悬至1×105/mL,取100μL,经股动脉将4T1luc

细胞注射到BALB/c雌鼠,并通过活体成像检验此方法转移成瘤的特异性.将100μL

相同浓度的RM-1细胞悬液分别经股动脉、髂外动脉注射C57雄鼠各15只,比较

两种不同注射方式所需时间、小鼠存活率,注射21天后将下肢骨HE切片,显微镜下

观察并比较两种方法建立骨转移模型的成瘤率.结果经股动脉途径注射肿瘤细胞悬

液所需的止血时间为2.53±1.75min,经髂外动脉注射方法所需的止血时间为

4.70±1.63min(P0.05);经股动脉注射方法的手术时间为14.67±2.16min,经髂

外动脉注射方法的手术时间为22.47±3.50min(P0.05);术后21d经股动脉和髂

外动脉注射组小鼠的生存率分别为93.3%和66.7%(P0.05);采用两种方法的肿瘤

转移率均为100%;分别经股动脉和髂外动脉注射生理盐水组和肿瘤细胞悬浮液组

在上述3项指标无显著差异