乳腺癌中Wnt信号通路成分NKD1、β-catenin和Cyclin D1蛋白的表达及意义.pdfVIP

乳腺癌中Wnt信号通路成分NKD1、β-catenin和Cyclin

D1蛋白的表达及意义

邹娜;金波;王红龙;刘天卿

【摘要】ObjectiveToinvestigatetheclinicalsignificanceofNKDl,β-

catenin,CyclinD1proteinexpressioninbreastcarcinoma.MethodsThirty

normalbreasttissuesand124non-specificinvasivebreastcancertissues

werecollectedfromDalianMunicipalWomanandChildrensMedical

CenterandDalianFriendshipHospital.Immunohistochemistrywasusedto

detecttheexpressionsofNKD1,β-catenin,CyclinD1protein.Spssl7.0

softwarewasusedforstatisticalanalysis.ResultsInbreastcancer

tissues,thenegativefrequencyofNKD1expressionwas69.35％,the

abnormalexpressionfrequencyofβ-cateninwas72.58％,andthepositive

frequencyofCyclinD1expressionwas54.03％.Thereweresignificant

differencesbetweennormalbreasttissuresandbreastcancer

tissues.Down-regulationofNKD1expressionandabnormalexpressionof

β-cateninweresignificantlycorrelatedwithhistologicgrades,pTNMstages

andthelymphnodemetastasisofbreastcancer(P＜0.05).Up-regulation

ofCyclinD1wassignificantlycorrelatedwithpTNMstagesandthelymph

nodemetastasisofbreastcancer(P＜0.05).NKD1expressionwas

positivelycorrelatedwithβ-cateninabnormalexpressionandCyclinD1

expression(r=0.454,r=0.332,P＜0.05).ConclusionsNKD1,β-catenin,Cyclin

D1proteincouldbecomeindicatorsofprognosisinbreastcancer.%目的探

讨NKD1、β-catenin和CyclinD1在乳腺癌中的表达及意义.方法选取2010年

至2016年大连市妇女儿童医疗中心和大连市友谊医院病理科30例正常乳腺组织

和124例乳腺浸润性癌非特殊型标本及临床资料.采用免疫组化法检测标本中

NKD1、β-catenin和CyclinD1蛋白的表达,并分析其与临床病理参数之间的关系.

结果在乳腺癌组织中NKD1阳性表达率为30.65％;β-catenin异常表达率为

72.58％;CyclinD1阳性表达率为54.03％,与正常乳腺组织比较,差异均有显著性意

义(P均＜0.05).NKD1和β-catenin表达与乳腺癌组织学分级、pTNM分期及淋

巴结转移有关,CyclinD1表达与乳腺癌pTNM分期及淋巴结转移亦有关,且NKD1

表达缺失还与ER状态相关,差异均有统计学意义(P均＜0.05).NKD1与β-catenin

和CyclinD1表达呈正相关(r分剐为0.454,0.332,P均＜0.05).结论N