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【动物医学论文】探讨防治应激条件下大鼠哮喘的作

用

［论文摘要］目的：探讨心理因素对哮喘发病的影响和调肝法调节应激、

防治哮喘的作用机制。方法：用卵蛋白加束缚应激刺激建立应激条件下

哮喘大鼠模型，采用放射免疫分析法、酶联免疫吸附法、原位杂交、免

疫组化、光镜及电镜等方法，检测各组大鼠血浆皮质酮、白细胞介素4、

干扰素γ、海马糖皮质激素受体mRNA和下丘脑促肾上腺皮质激素释放

激素（adrenocorticotropichormonecorticoliberin,CRH）阳性神

经元，并观察肺和海马组织形态学的变化。结果：疏肝理肺方可明显降

低血浆皮质酮水平，升高血浆干扰素γ，降低血浆白细胞介素4，减少

下丘脑室旁核CRH阳性神经元，上调海马CA3区糖皮质激素受体mRNA

的表达，减轻海马神经元的损伤，并可明显减轻应激条件下哮喘大鼠肺

组织的病理改变。结论：疏肝理肺方通过抑制应激条件下哮喘大鼠下丘

脑垂体肾上腺皮质轴功能的亢进及调节免疫系统的功能，从而起到防治

哮喘的作用。

［论文关键词］应激;哮喘;疏肝理肺法

ProtectiveandtherapeuticeffectsofShuganLifeiRecipeon

ratswithasthmaunderstressandthemechanisms

ABSTRACTObjective:Toexploretheinfluenceof

psychologicalfactoronasthma,theprotectiveandtherapeutic

effectsofShuganLifeiRecipeonratswithasthmaunderstress

andthemechanisms.Methods:Allergyandstressinducedasthma

modelswereestablishedinratsbygivingovalbuminand

restrainingstress.Radioimmunoassay,enzymelinked

immunosorbentassay,insituhybridizationand

immunohistrochemicalmethodwereusedtodetectplasma

corticosterone,IL4,IFNγ,expressionofglucocorticoid

receptor(GR)mRNAinhippocampusandadrenocorticotropichormone

corticoliberin(CRH)positveneuroninhypothalamus.Opticaland

electronmicroscopeswereusedtoobservethemorphological

changesofpulmonaryhilarandhippocampaltissues.Results:

ShuganLifeiRecipe(SGLFR)couldreduceplasmacorticosterone,

decreaseCRHpositiveneuronsinparaventricularnucleusof

hypothalamus,upregulatetheexpressionofGRmRNAinhippocampus

andreducetheinjuryofhippocampalneuron.SGLFRhadcertain

inhibitiveeffectonhyperfunctionof

hypothalamopituitaryadrenalcortex(HPA)axisinratswithasthma

understressandcouldalsorelievethepathologicalchangesof

thepulmonaryhilartissue.ThelevelofplasmaIFNγwas

increasedwhilethelevelofplasmaIL4wasdecreasedin

SGLFRtreatedgroup.Conclusion:T