肝癌中烯酯酰辅酶A水解酶1的表达及其亚细胞定位 .pdfVIP

肝癌中烯酯酰辅酶A水解酶1的表达及其亚细胞定位

张军;曲鸿;毛俊;魏元怡;黄玉红;王华新;唐建武

【摘要】目的：探讨烯酯酰辅酶A水解酶1(enoylcoenzymeAhydratase1,

Ech1)表达变化在肝癌中的意义及Ech1的亚细胞定位与肿瘤恶性生物学行为的关

系。方法应用免疫组化法检测20例正常肝组织和30例原发性肝癌组织中Ech1

的表达；应用亚细胞分离法检测Ech1在Hca-F和FEch1下调细胞的四种不同亚

细胞组分中的表达。结果Ech1在原发性肝癌中的表达高于正常肝组织,差异有统

计学意义(P0．05)；Ech1表达与患者年龄、AFP值及是否伴有肝硬化或伴有病

毒性肝炎差异无统计学意义(P0．05)。Ech1在Hca-F和FEch1下调细胞的四

种亚细胞组分中表达均定位于细胞质和细胞膜,并且细胞质表达强于细胞膜,细胞核

和细胞骨架部分中不表达。结论Ech1在肝癌组织中的表达明显高于正常肝组织,

提示其可能与肝癌的发生、发展有关；体外培养细胞中Ech1表达调控对其亚细胞

定位的改变没有影响,Ech1对肿瘤恶性生物学行为的影响可能不是通过其亚细胞定

位改变而实现的。%PurposeTostudytheexpressionofEch1in

hepatocarcinomaanditsclinicalsignificance,andtoexplorethe

relationshipbetweensubcellularlocationofEch1andmalignantbiologic

behaviourofhepatocarcinoma.MethodsImmunohistochemicalanalysis

wasusedtodetectEch1expressioninthe20casesofnormallivertissue

andthe30casesofhepatocarcinoma.SubcellularlocationofEch1inHca-F

andFEch1-downcellwasobservedwithsubcellularproteinextraction.

ResultsTheexpressionofEch1inprimaryhepa-tocarcinomawasincreased

comparedtothatofnormallivertissues(P0.05),whiletherewasno

significantdifferencebetweentheexpressionofEch1withtheage,AFP,

andwithorwithoutlivercirrhosisorhepatitisvirusinhepatocarcinoma

(P0.05).Ech1wasfoundexpressedalmostatthesamelocationalthough

theexpressionleveloneisnormalandtheotherisdownregulation.Ech1

expres-sionwasfoundincytosol,membranefraction,anditsexpression

washigherincytosolthanotherfractionsbothinHca-FcellandEch1

downregulatedHca-Fcell.ConclusionsTheexpressionofEch1inprimary

hepatocarcinomawasincreased,whichmayindicatethatEch1isacritical

factorinthedevelopmentofprimaryhepatocarcinoma,butthesubcellular

locationofEch1hasnotmuchcontributiontothat.

【期刊名称】《临床与实验病理学杂志》

【年(卷),期】2015(000)003

【总页数】4页(P247-250)

【关键词】肝肿瘤;Ech1;亚细胞定位

【作者】张军;曲鸿;毛俊