肺癌模型小鼠肿瘤和主要免疫器官组织中调节性 T 细胞和NK 细胞的数量变.pdfVIP

肺癌模型小鼠肿瘤和主要免疫器官组织中调节性T细胞和

NK细胞的数量变化及其意义

郑全辉;王娜;宋天姣;曹倩文;田雨;郝小惠;张爱红

【摘要】Objective:ToexplorethechangesofnumberofCD4+CD25+

foxp3+regulatoryTcells(Treg)andnaturalkillercells(NK)inthe

peripheralimmuneorgansandtumortissueofthemurinemodelsoflung

cancer,andtoclarifytheireffectsonthedevelopmentoflung

cancer.Methods:TheC57BL/6miceweredividedintoLewislungcarcinoma

cells(LLC)injectiongroupandnormalcontrolgroup.ThemiceinLLC

injectiongroupwereinjectedwithLLCsubcutaneouslyinthearmpitto

establishthetumormodels,whilethemiceinnormalcontrolgroupwere

injectedwiththesameamountofsaline.ThenumberofCD4+CD25+T

cells,CD4+CD25+foxp3+Tregsinthespleen,lymphnodesandlung

cancertissues,andthenumberofNKcellsinthespleentissuewerelabeled

bycellsurfaceorintracellularantibodystaining,anddetectedbyflow

cytometry.Results:TheratiosofCD4+CD25+TcellstoCD4+T

cells,foxp3+cellstoCD4+CD25+Tcells,andthenumberofCD4+CD25+

foxp3+Treginthenbsp;spleenandlymphnodesofthemiceinLLC

injectiongroupwereincreasedsignificantlycomparedwithnormalcontrol

group(P0.05orP0.01).Moreover,theratiosofCD4+CD25+Tcellsto

CD4+Tcellsandfoxp3+cellstoCD4+CD25+Tcellsinthetumortissue

weresignificantlyhigherthanthoseinthespleenandlymphnodesofthe

miceinLLCinjectiongroup.However,theratioofNKcellsinthespleen

tissueofthemiceinlungcancergroupwassignificantlydecreased

comparedwithnormalcontrolgroup(P0.05).Conclusion:Theincreaseof

ratioandthenumberofTregcellsandthedecreaseofratioofNKcellsin

themainimmuneorgansoflungcancermicemaypromotethe

developmentoftumorandinhibittheimmuneresponsetocancercellsin

vivo.%目的：探讨肺癌小鼠CD4＋CD25＋Foxp3＋调节性T细胞（Treg）和

自然杀伤细胞（NK）在外周免疫器官和肿瘤组织中数量的变化，阐明其对肿瘤发

展的影响。方法：C57BL／6小鼠分为Lewis肺癌细胞系（LLC）注射组