circEGFR通过miR-106a-5p-DDX5轴促进结直肠癌细胞的增殖和迁移.pdfVIP

circEGFR通过miR-106a-5p-DDX5轴

促进结直肠癌细胞的增殖和迁移

摘要：circEGFR是一种环状RNA，在许多癌症中都表现出高表

达水平，而在结直肠癌中也不例外。本文研究发现circEGFR

通过miR-106a-5p/DDX5轴促进结直肠癌细胞的增殖和迁移。

首先，在结直肠癌细胞中，circEGFR表现出高表达水平，并

通过荧光原位杂交和RNaseR消化实验证明了其环状结构。接

着，实验室发现circEGFR通过miR-106a-5p的调节增强了结

直肠癌细胞的增殖和迁移。最后，通过对细胞周期和凋亡相关

基因进行荧光定量PCR和WesternBlot实验，本研究确定

DDX5是miR-106a-5p的靶点，circEGFR通过调节DDX5的表达

进一步促进了结直肠癌细胞的增殖和迁移。因此，本研究揭示

了circEGFR通过miR-106a-5p/DDX5轴调控结直肠癌细胞生长

和迁移的分子机制，为结直肠癌的研究提供了新的方向和治疗

靶点。

关键词：结直肠癌；circEGFR；miR-106a-5p；DDX5；增殖；

迁移

Abstract:CircEGFRisacircularRNAthatis

frequentlyoverexpressedinmanytypesofcancer,

includingcolorectalcancer.Inthisstudy,we

investigatetheroleofcircEGFRinpromotingthe

proliferationandmigrationofcolorectalcancercells

throughitsregulationofthemiR-106a-5p/DDX5axis.

WeshowthatcircEGFRishighlyexpressedin

colorectalcancercellsandthatitpossessesa

circularstructure,asevidencedbyfluorescencein

situhybridizationandRNaseRdigestionassays.We

demonstratethatcircEGFRenhancestheproliferation

andmigrationofcolorectalcancercellsthroughits

regulationofmiR-106a-5p.Furthermore,weidentify

DDX5asatargetofmiR-106a-5panddemonstratethat

circEGFRpromotestheproliferationandmigrationof

colorectalcancercellsbyregulatingtheexpression

ofDDX5.Ourfindingsrevealthemolecularmechanism

bywhichcircEGFRpromotesthegrowthandmetastasis

ofcolorectalcancercellsthroughthemiR-106a-

5p/DDX5axis,providingnewinsightsandpotential

therapeutictargetsforthetreatmentofcolorectal

cancer.

Keywords:colorectalcancer;circEGFR;miR-106a-5p;

DDX5;proliferation;migratio。

Introduction:

Colorectalcancerisacommonmalignanttumorinthe

digestivesystem.Althoughgreatprogresshasbeen

madeinthediagnosisandtreatmentofcolorectal

cancer,themechanismsunderlyingitspathogenesisand

progressionremainunclear.CircularRNAs(circRN