急性髓系白血病CDX2和β-catenin基因表达的相关性 .pdfVIP

急性髓系白血病CDX2和β-catenin基因表达的相关性

王巍;孟灿;刘佳;杨志刚

【摘要】ObjectiveToinvestigatetheexpressionsofCDX2andβ-catenin

andtheircorrelationinacutemyeloidleukemia(AML).MethodsReal-time

PCRwasusedtoexaminetheexpressionsofCDX2andβ-cateninmRNAin

92denovoAMLand30non-malignantpatients(control),andWestern

blotwasemployedtodetectCDX2andβ-cateninproteinexpressionsin26

denovoAMLand8non-malignantpatients.ResultsThepositivityratesof

CDX2mRNAandproteinexpressionsinAMLgroupwere84.78%and

76.92%,respectively,butnoCDX2mRNAorproteinexpressionwere

detectedincontrolgroup(P0.01).Theexpressionsofβ-cateninmRNA

andproteinweredetectedinallthepatientsinbothgroupsbutits

expressionsinAMLgroupweresignificantlyhigher(P0.01).The

expressionsofCDX2andβ-cateninmRNAweresignificantlycorrelated

withWBCcountsandLDHlevels(P0.01).TheexpressionofCDX2mRNA

wasnotdetectedandtheexpressionofβ-cateninmRNAwasdecreasedto

normallevelinAMLpatientswithcompleteremission,whilethe

expressionsofCDX2andβ-cateninmRNAwereincreasedagaininthose

withdiseaserelapse.Thereweresignificantlypositivecorrelationsbetween

CDX2andβ-cateninexpressionsatbothmRNAandproteinlevels(P0.01).

ConclusionUp-regulationofCDX2geneandactivationofWnt/β-catenin

pathwayco-existinAMLpatientsandtheexpressionsofCDX2andβ-

cateninarepositivelycorrelated.%目的：研究急性髓系白血病（AML）患者

CDX2与β-catenin基因表达的相关性。方法实时定量PCR方法检测92例初诊

AML、30例非恶性血液病患者（对照）CDX2及β-cateninmRNA表达，

Westernblot检测其中的26例AML、8例非恶性血液病患者CDX2、β-catenin

蛋白表达。结果AML组CDX2mRNA和蛋白表达阳性率分别为84.78%和

76.92%，对照组未检测到表达（P0.01）；β-cateninmRNA和蛋白在两组均

表达，但AML组显著高于对照组（P0.01）。CDX2、β-cateninmRNA表达均

与初诊时白细胞数、LDH水平呈正相关（P0.01）。AML患者获得完全缓解时，

CDX2mRNA转阴、β-catenin