GSK-3β、PTEN 、PLK1在儿童急性髓系白血病中的表达及意义 .pdfVIP

GSK-3β、PTEN、PLK1在儿童急性髓系白血病中的表达及

意义

王妍;徐酉华;胡艳妮;孙艳辉;金鑫;杨桂存

【摘要】ObjectiveToexploretheexpressionandclinicalsignificanceof

GSK-3β,PTENandPLK1inpediatricAML.MethodsExperimentgroupwas

bonemarrowsof33casesincipientchildrenwithAML.Controlgroupwas

10casesnormalbonemarrows.GSK-3β,PTENandPLK1geneexpressions

inBMMNCofthetwogroupsweretestedusingsemiquantitativereverse

transcriptasepolymerasechainreaction(RT-PCR)technique.GSK-3βprotein

andP-GSK-3βexpressionsweretestedbyELISA.Re-sultsTheexpressionof

GSK-3βmRNA,GSK-3βprotein,PLK1mRNAinBMMNCofchildrenwith

AMLwashigherthanthatofcontrolgroup(P=0.012;P=0.014;P=

0.040);TheexpressionofPTENmRNA,P-GSK-3βwaslowerthanthe

controlgroup(P=0.012;P=0.002);GSK-3βproteinhadanegative

correlationwithPTENmRNA(r=-0.415,P=0.016);GSK-3βmRNA,GSK-

3βproteinhadapositivecorrelationwithPLK1

mRNA(r=0.388,P=0.026;r=0.427,P=0.013).TheexpressionofGSK-

3βproteinwashighinwhichhadhighperipheralwhitebloodcell

counts;boththeexpressionsofGSK-3βmRNAandGSK-3βpro-teinwere

highinwhichhadhighrisk;buttheexpressionofP-GSK-3βwere

low.ConclusionInpediatricAML,GSK-3βandPLK1mayplayaroleof

oncogeneandPTENmayplayaroleoftumorsuppressorgene.%目的：探

讨糖原合酶激酶-3β（GSK-3β）、与张力蛋白同源的位于第10号染色体磷酸酶基

因（PTEN）、Polo样激酶1（PLK1）在儿童急性髓系白血病（AML）中的表达

及意义。方法RT-PCR方法检测实验组（33例初诊AML患儿的骨髓）和对照组

（10例正常骨髓）骨髓单个核细胞（BMMNC）中GSK-3βmRNA、PTEN

mRNA、PLK1mRNA的表达，ELISA方法检测GSK-3β蛋白及P-GSK-3β的表

达。结果实验组中GSK-3βmRNA、GSK-3β蛋白、PLK1mRNA的表达量高于

对照组（P＝0．012；P＝0．014；P＝0．040）；PTENmRNA、P-GSK-3β的

表达量低于对照组（P＝0．012；P＝0．002）。GSK3β蛋白与PTENmRNA的

表达呈负相关（r＝-0．415，P＝0．016）；GSK-3βmRNA、GSK3β