苦参水提物治疗湿疹豚鼠皮肤炎症的实验研究 .pdfVIP

苦参水提物治疗湿疹豚鼠皮肤炎症的实验研究

陈燕;张丽华

【摘要】目的通过分析苦参水提物对湿疹豚鼠皮肤炎症肿胀度的抑制作用,并观察

病理变化,探讨其抗炎作用,为临床治疗提供实验依据.方法豚鼠40只,用2,4-二硝

基氯苯在豚鼠颈背部涂抹致敏,在右耳内侧激发造模.随机分成5组,每组8只,分别

作为模型对照组、阳性对照组、苦参提取液高、中、低浓度组,对湿疹部位皮肤连

续涂抹给药14天,取双耳等面积的同部位,以左、右耳重量之差来评价皮肤炎症肿

胀度.并取右耳组织块,HE常规染色,制作组织病理切片,观察其病理变化.结果与模

型对照组相比,苦参水提物高、中浓度组豚鼠皮肤肿胀度明显减轻(p0.05),且真皮

水肿和炎性细胞浸润情况明显改善(p0.05);与阳性对照组比较,苦参水提物高浓度

组的豚鼠皮肤肿胀度、真皮水肿和炎性细胞浸润情况亦明显减轻(p0.05).结论

苦参水提物能有效减少湿疹豚鼠皮肤的炎症反应,对于开拓湿疹的临床治疗方法具

有一定的研究意义.%ObjectiveToinvestigatetheinhibitionofSophora

flavescensextractonthedegreeofinflammatoryswellingofguinea

pigsskinwitheczema,observepathologicalchangesandexplorethe

effectsoftheanti-in-flammatoryaction,inordertoprovideexperimental

basisforclinicaltreatment.MethodsEczemamodelsof40guineapigs

weresetupbysmearing2,4-dinitrochlorobenzeneontheirnapestocause

sensitizationandmotivatingintheinsideoftheirrightears.Allguineapigs

wererandomlydividedinto5groupsequally,includingthemodelcom-

paredgroup,positivecontrolgroupandhigh,medium,lowconcentration

groupsofsophoraflavescens.Aftereczemaskinwascontinuously

administereddrugsfor14days,thesamepartwithequalareaoftheleft

andrightearswastakenandweighed.Thedifferencebetweentheleftand

rightearswasusedtoevaluatetheswellingdegreeofskin

inflammation.Thepathologicalchangeswereobservedbymaking

pathologicalsliceswithHEstainingwiththetissueblocksofright

ears.ResultsComparedwiththemodelgroup,theswellingdegreeofskin

inflammationwassignificantlyrelievedanddermaledemaand

inflammatorycellinfiltratingweresignificantlyimprovedinthehighand

medi-umconcentrationgroupsofsophoraflavescens(p0.05).Compared

withthepositivegroup,theswellingdegreeofskininflammation,dermal

edemaandinflammatorycellinfiltratingweresignificantlyalleviatedinthe

highconcentra-tiongroupofsophoraflavescens(p0.05)