雌二醇及中波紫外线在豚鼠皮肤色素沉着形成中的作用及其机制.pdfVIP

雌二醇及中波紫外线在豚鼠皮肤色素沉着形成中的作用及其机

制

周颖丽;宋秀祖;洪为松;相文忠

【摘要】ObjectiveToinvestigatetheinfluenceofestradiolandultraviolet

B(UVB)radiationonmelaninme-tabolismofguinea-pig’sbrowndorsal

skin,andexploretherelationshipbetweentheestrogenandultravioletin

skinpigmentarydisorder.MethodsHealthysuitguinea-pigswereselected

inthisstudy.Theguinea-pigsskinwasdi-videdintosolventcontrolgroup,

UVBirradiationgroup,estradioltreatmentgroupandUVBirradiationafter

estradi-olpertreatmentgroup(estradiol+UVBgroup).The

concentrationsofestradiolweresetto3.67×10-5,3.67×10-4,3.67×

10-3mol/L.TheUVBexposuredosewasunifiedas500mJ/cm2.Invivo

confocallaserscanningmi-croscopy(referredtoasskinCT)and

transmissionelectronmicroscopywereusedtoobservetheeffectsof

estradiolandUVBonmelaninmetabolismanddegradation.Results①The

pigmentationofguinea-pigskinwasdeepenedafteracertain

concentrationofestradioltreatment,increasedgraduallywiththe

increasingofestradiolconcentration(P0.05).②Thepigmentationof

guinea-pigskinwasalsodeepenedsignificantlyinUVBirradiationgroup,

comparedwithnon-UVBirradiationgroup(P0.05).③Theautophagyof

epidermalkeratinocytesofGuineapigsincreasedwithUVBirradiation,but

decreasedwithestradioltreatment.ConclusionEstradiolandUVB

irradiationcanpromotepigmentationofguinea-pigskinandthe

mechanismmayberelatedtothepromotionofmelanosomessynthesis.

Thedegradationofabnormalmelanosomesmayalsobeinvolvedinthe

formationofpigmentation.%目的：研究雌二醇和中波紫外线(UVB)辐射对豚鼠

背部棕色皮肤中黑素代谢的影响，探讨雌激素及UVB在皮肤色素沉着中的作用。

方法选取健康花色豚鼠，设立溶媒对照组、UVB照射组、雌二醇处理组和雌二醇

预处理后UVB照射组(雌二醇+UVB组)；雌二醇浓度设为3．67×10-5、

3．67×10-4、3．67×10-3mol/L；UVB照射剂量统一为500mJ/cm2。采用皮

肤在体共聚焦激光扫描显微镜(简称皮肤CT)以及透射电子显微镜等观察雌二醇和

UVB对黑素小体合成及降解的影响。结果①一定浓度雌二醇可加深豚鼠皮肤色素

沉着，且随着雌二醇浓度的递增，沉着程度逐渐