致畸试验中3种常用阳性对照物的效果比较.pdfVIP

致畸试验中3种常用阳性对照物的效果比较

李培宁;江漪;陈秀娟;刘香梅;庞增雄;黄宇锋

【摘要】目的:比较致畸试验中3种常用阳性对照物的致畸效应,为食品、保健品、

化妆品致畸试验中阳性对照物的选择提供参考.方法:将交配成功的SD大鼠按照妊

娠时间随机分为4组,分别为阴性对照组,阿司匹林组(280mg/kg),敌枯双组(1

mg/kg)和环磷酰胺组(12mg/kg).阿司匹林组和敌枯双组于受孕后第6~15天按

10mL/kg分别灌胃相应的溶液,环磷酰胺组于受孕后第12天腹腔一次注射给药.记

录受孕后第0、6、9、12、15、20天孕鼠体质量.于受孕后第20天麻醉处死孕鼠,

迅速取出子宫,称子宫连胎质量及子宫质量,记录黄体数、着床数及胚胎发育情况.对

胎仔进行外观检查,逐一记录性别、体质量、体长.将每窝1/2活胎鼠染色做骨骼检

查,另外1/2活胎鼠固定做内脏检查.结果:与阴性对照组比较,阿司匹林组孕鼠第15、

20天体质量及体质量增量降低,子宫、子宫连胎质量降低,活胎率减少,活胎鼠体质

量、体长、尾长均降低(P0.05或0.01);敌枯双组活胎率降低,活胎鼠体质量、体

长、尾长均降低(P0.01);环磷酰胺组孕鼠体质量增量降低,子宫、子宫连胎质量降

低,活胎率减少(P0.05或P0.01);3种阳性对照物处理组大鼠胎仔外观畸形率、

骨骼畸形率及内脏畸形率明显升高(P0.01),以敌枯双组和环磷酰胺组畸形率较高,

畸胎类型多.结论:在本试验条件下,可见敌枯双(1mg/kg)和环磷酰胺(12mg/kg)处

理组大鼠的畸形率高,畸胎类型多,是较为敏感的、致畸类型较全面的阳性对照

物.%OBJECTIVE:Toprovidepositivereferenceforteratogenicityteston

food,healthcareproductsandcosmetics,weexploredteratogeniceffects

ofthreedifferentpositivecontrolmaterials.METHODS:Accordingtothe

gestationtimeofSDrats,pregnantratswererandomlydividedinto

control,aspirin(280mg/kg),Bis-A-TDA(1mg/kg)andCP(12mg/kg)

groups.Theirbodyweightsondays0,6,9,12,15and20ofpregnancywere

recorded.Onday6-15ofgestation,theaspirinandBis-A-TDAgroupswere

administratedcorrespondingsolutions,whileCPgroupwereinjectedip

withCPonday12.Pregnantratswereinspectedbycesareansectionand

weightsofuterus,uterineandfetuseswererecorded.Thenumberof

corpusluteum,implantationpoint,andembryonicdevelopmentwere

examined.Appearanceoffetuswasexamined,andfetalweight,bodylength

andtaillengthofeachfetusweremeasured.1/2ofthelivefetuswere

stainedforskeletalexaminationandtheotherswerefixedforvisceral

examination.RESULTS:Intheaspiringroup,bodyweightandweight

incrementofthepregnantrats,theweightoftheuterusanduterineand

fetalwerereduced.Theratesoflivefetus,fetalweight,thelengthofbody

andtailweredecr