枸杞多糖对β淀粉样蛋白1-40致PC12细胞线粒体损伤的影响.pdfVIP

枸杞多糖对β淀粉样蛋白1-40致PC12细胞线粒体损伤的

影响

成江;于成超;侯晓霖;李芳;杨娟;李海宁

【摘要】目的探讨枸杞多糖(LBP)对β淀粉样蛋白(βamyloidpeptide,Aβ1-40)致

PC12细胞线粒体损伤的作用.方法对数生长期的PC12细胞分为5组:对照组、模

型组、LBP高、中、低剂量组(40、20和10mg·L-1).Aβ1-4040μg·mL-1处理细

胞24h为模型组.LBP高、中、低剂量分别预处理PC12细胞24h,加入β1-

4040μg·mL-1继续孵育24h.显微镜下观察细胞形态,CCK8法检测细胞存活率,分

光光度计检测细胞内活性氧簇(ROS),激光共聚焦显微镜检测线粒体膜电位(MMP)

及观察线粒体形态.结果模型组细胞状态不良,与对照组比较,模型组细胞存活率降

低,ROS显著升高,MMP明显降低(P均0.01),线粒体形态呈颗粒状;与模型组比

较,LBP高、中、低剂量组可改善细胞形态,提高细胞存活率(P0.05或0.01),降低

ROS(P0.05或0.01)及显著升高MMP(P0.01),线粒体形态改善,呈管网状结构.

结论LBP对Aβ1-40诱导损伤的PC12细胞线粒体有保护作用.%ObjectiveTo

investigatetheprotectiveeffectsofLyciumBarbarum

Polysaccharide(LBP)onmitochondrialdysfuctionofPC12cellsinjuredby

Aβ1-40.MethodsPC12cellsweredividedinto5groups:control

group,modelgroupandLBPgroups(10,20,40mg·L-1).Modelgroupwas

treatedwithAβ1-4040μg·mL-1for24h.LBP(10,20,40mg·L-1)weregiven

24hpriortoAβ1-4040μg·mL-1incubationandmaintainedfor24hpost-

Aβ1-40treatment.Aftertreatments,CCK8assaywasemployedtoanalyze

thePC12cellviability.Thelevelsofreactiveoxygenspecies(ROS)inPC12

cellswasmeasuredbyspectrophotometer.Confocallaserscanning

microscopewasappliedtodetectmitochondrialmembrane

potential(MMP)andtoobservemitochondrialmorphologywith

MitoTrackerRedCM-Xros.ResultsAβ1-40inducedadecreasein

viability(P0.01)andanincreaseofROSinPC12cells(P0.01)alongwith

dissipationofMMP(P0.01).Aβ1-40ledtomorphologicalchangesin

mitochondria.PretreatmentwithLBP(10,20,40mg·L-1)significantly

increasedthecellviability(P0.05).Furthermore,LBPinhibitedsignificantly

theoverproductionofROSanddissipationofMMP(P0.01).Mitochondrial

morphologywasimprovedbyLBP.ConclusionLBPshownneuroprotective

effectsonPC12cellsdamagedbyAβ1-40.

【期刊名称】《宁夏医科大学学报》

【年(卷),期】2016(038)012

【总页数】5页(P1371-1374,封4)

【关键词】枸杞多糖;β淀粉样蛋白;阿尔茨