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SequenceAnalysisViewer(SAV):ABeginner’sGuide
RuipingDeng
TechnicalSupport|07/30/2020
QB#7279
©2020Illumina,Inc.Allrightsreserved.ForResearchUseOnly.Notforuseindiagnosticprocedures.
NOTE:Thismaterialisintendedforbeginners.AdditionaladvancedwebinarsonusingSequenceAnalysisViewer(SAV)fortroubleshootingrunsonspecificIlluminaSequencingplatformsareavailable.ThiswebinarisanintroductorycourseonSAV.Wewillnotbegoingintoin-depthtroubleshootingscenarios,butinsteadwillfocusonhowtouseSAV.
/science/education/support_audio1.html
2
Objectives
➢LearnhowtoloaddatainSequenceAnalysisViewer(SAV)
➢LearnwhatinformationisavailableineachtabofSAV
➢EnableruntroubleshootingthroughanunderstandingofSAVdata
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SequenceAnalysisViewer(SAV)isanapplicationthatallowsyoutoviewimportantqualitymetricsgeneratedbytheRealTimeAnalysissoftware(RTA)onIlluminasequencingsystemsduringarunandafterithascompleted.
SequenceAnalysisViewer
4ForResearchUseOnly.Notforuseindiagnosticprocedures.
SAVdisplaysthesamemetrics,customizedfordifferentplatforms
ForResearchUseOnly.Notforuseindiagnosticprocedures.
HiSeqTM2500HighOutput
HiSeqTM2500RapidRun
HiSeqTM3000/4000
NovaSeqTM6000
iSeqTM100
MiniSeqTM
MiSeqTM
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Loadingarun
Analysis
Tab
ImagingTab
SummaryTab
➢QscoreDistribution
➢DatabyCycle
➢FlowCellChart
➢DatabyLane
➢QscoreHeatmap
➢ThumbnailImages
➢Drop-DownFields
➢DataFilters
➢PlottingData
➢FilesRequired
➢Howtoopenarun
SAV101Outline
Datalane
Dataread
➢Sampleindices
per
per
➢
➢
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Loadingarun
Analysis
Tab
ImagingTab
SummaryT
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