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WelcomeEachofYoutoMyMolecularBiologyClass?
MolecularBiologyoftheGene,5/E---Watsonetal.(2004)PartI:ChemistryandGeneticsPartII:MaintenanceoftheGenomePartIII:ExpressionoftheGenomePartIV:RegulationPartV:Methods3/08/05?
PartII:MaintenanceoftheGenomeDedicatedtothestructureofDNAandtheprocessesthatpropagate,maintainandalteritfromonecellgenerationtothenext?
MaintenanceoftheGenomeCh6:ThestructuresofDNAandRNACh7:Chromosomes,chromatinsandthenucleosomeCh8:ThereplicationofDNACh9:ThemutabilityandrepairofDNACh10:HomologousrecombinationatthemolecularlevelCh11:Site-specificrecombinationandtranspositionofDNA3/08/05?
CHAPTER6TheStructuresofDNAandRNAHowdothestructuresofDNAandRNAaccountfortheirfunctions??
OUTLINE1.DNAStructure2.DNATopology3.RNAStructure?
DNASTRUCTURE?
DNASTRUCTURE(1)DNAiscomposedofpolynucleotidechainsStructure:twistingaroundeachotherintheformofadoublehelix.?
SchematicmodelSpace-fillingmodel?
NucleosideNucleotide,thefundamentalbuildingblockofDNAglycosidicbondphosphoesterbond?
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DNApolarity:isdefinedbytheasymmetryofthenucleotidesandthewaytheyarejoined.Phosphodiesterlinkages:repeating,sugar-phosphatebackboneofthepolynucleotidechain?
BasesinDNApurinespyrimidinesadenineguaninecytosinethymineN9N1?
DNASTRUCTURE(2)Eachbaseshasitspreferredtautomericform(RelatedtoCh9)?
Thetwostrandsofthedoublehelixareheldtogetherbybasepairinginanantiparallelorientation,Whichisastereochemicalconsequenceofthewaythatadenineandthymine,andguanineandcytosine,pairwitheachother.(Relatedtoreplicationandtranscription)DNASTRUCTURE(3)?
TheTwoChainsoftheDoubleHelixHaveComplementarySequencesExample:Ifsequence5’-ATGTC-3’ononechain,theoppositechainmusthavethecomplementarysequence3’-TACAG-5’DNASTRUCTURE(4)Watson-CrickBaseP
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