超高效液相色谱-串联质谱法快速测定大鼠血清中8种神经递质 .pdfVIP

超高效液相色谱-串联质谱法快速测定大鼠血清中8种神经递

质

赵芳;李强;梁梅丽;闫艳;邢婕;秦雪梅;高晓霞

【摘要】建立了同时测定8种神经递质(5-HT、GABA、Glu、ACH、NE、DA、

5-HIAA和HVA)的超高效液相色谱-三重四极杆质谱方法(UHPLC-MS/MS),并用

于大鼠血清样品的测定.大鼠血清经含0.1%甲酸-乙腈沉淀蛋白处理后,选用

WatersACQUITYUPLCBEHC18色谱柱(100mm×2.1mm,1.7μm),以0.1%甲

酸-乙腈为流动相梯度洗脱进行分离;采用电喷雾离子源(ESI),在正离子扫描下,采用

多反应监测模式(MRM)对8种神经递质及内标化合物进行检测.结果表明,8种神经

递质可在10min内准确测定;定量限为1.8ng/mL,且线性良好,相关系数均大于

0.994,日内、日间精密度(n=6)≤9.2%,稳定性、加标回收率和基质效应等均符合分

析要求.本方法分析时间短、准确度好、灵敏度高、专属性强、稳定性好、基质效

应小,适用于血清中3类(单胺类、氨基酸类和乙酰胆碱)共8种神经递质的同时定

量检测.%Anultra-highperformanceliquidchromatographytandemmass

spectrometric(LC-MS/MS)methodforsimultaneousdeterminationof8

kindsofneurotransmitters(5-HT,GABA,Glu,ACH,NE,DA,5-HIAA,HVA)in

ratserumwasdeveloped.Thebloodsampleswereextractedby0.1%

formicacidinacetonitrileandseparatedonaWatersACQUITYUPLCBEH

C18(2.1mm×100mm,1.7μm)usinggradientelution,withthemobile

phaseconsistingofacetonitrileand0.1%formicacidinwater.Thesamples

werethenioniZedwithpositiveelectrospray(ESI+),anddetectedunder

multiplereactionmonitoring(MRM)mode.Asaresult,8kindsof

neurotransmittersweredeterminedaccuratelyin10minwithalimitof

quantitationof1.8ng/mLandintra-dayandinter-dayprecisionsof≤9.2%

(n=6).Thismethodshowedagoodlinearityindetectionof

neurotransmittersandthelinearcorrelationcoefficientsweregreaterthan

0.994.Alsothestability,recoveryandmatrixeffectwereeligibleforthe

analysis.Thismethodshowedhighaccuracy,sensitivity,strongspecificity,

goodstability,smallmatrixeffectandshorttimeforanalysis,andwas

suitableforthequantitativedeterminationofmonoamine,aminoacids

andacetylcholineneurotransmittesinratserum.

【期刊名称】《分析化学》

【年(卷),期】2018(046)001

【总页数】8页(P121-128)

【关键词】超高效液相色谱-串联质谱;神经递质;血清;含量测定

【作者】赵芳;李强;梁梅丽;闫艳;邢婕;秦雪梅;高晓霞

【作者单位】山西大学中