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Chapter9-AnintroductiononElectrochemicalAnalysis1
Whatisinstrumentalanalysis?InstrumentsasthebasictoolsforanalysisVolumetricandgravimetric:classicanalysis2
Whygoesinstrumental?Efficiency:timeandthroughputEconomic:laborandcostSensitivity:ppm,ppt,evensinglemoleculeUnfavorable:relativelylargeerror,highcostinstrumentsrequired3
Whatkindofinformationwecanget?Qualitative:what’sthereQuantitative:howmuchStructurespatialdistribution4
Generalprocess5
6
Whatareincluded?ClassificationElectrochemicalanalysisChromatographicanalysisSpectrometricanalysis(atomicandmolecular)MassspectroscopyOthertechniques7
AshorthistoryStartfromtheearly20thcenturyDevelopmentofphysicalchemistryElectronicandphysics(1940’s)Computertechnology(1960~1970’s)InternetandArtificialIntelligence8
NewtrendsHigherperformances(sensitivity,selectivity,throughput,etc.)Combinationofvarioustechniques(e.g.LC-MS,)Fieldanalysis(on-line,in-situ,in-vivo,etc.)Automatic,artificialintelligenceVariousforms:bentchtop,laptop,handheld,imbedded9
Example:DNAsequencingforHGP1970’s,two-dimensionalchromatographyChain-terminationmethods(Sanger)Singlemoleculerealtimesequencing PacificBiosciencesexpectstocommercializeSMRTsequencingin2010or2011.TheprototypeoftheSMRTchipcontains~3000zero-modewaveguide(ZMW)holesthatallowparallelizedDNAsequencing.EachoftheZMWholesproducesapproximately1,500bp(basepair)readlengthsataspeedof10bppersecond.10
11
References12
ArrangementofourfollowingcourseIntroduction:CH01Electrochemicalanalysis:CH22~25Chromatography:CH26-30Atomicspectroscopy:CH06-10Molecularspectroscopy:CH13-2013
Electrochemicalanalysis
-anintroduction14
WhatiselectrochemicalanalysisMethodstoobtainqualitativeorquantitativeinformationthroughelectrochemicalandanalyticalchemistryprinciples15
What’sspecialinelectrochemicalanalysis?Highsensitivity,s
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