白腐真菌对石油污染土壤的修复研究.docx

化学工程师

Sum235No.04ChemicalEngineer2015年第04期

DOI:10.16247/ki.23-1171/tq白腐真菌对石油污染土壤的修复研究*

谭丽泉，余梅，周天*,彭镇宇

(广东石油化工学院化学工程学院，广东茂名525000)

摘·要：以原油为反应底物，利用白腐真菌作为降解菌，考察了土壤含水率、摇床转速、土壤含油率、Tween80及藜芦醇等因子对土壤中原油降解率的影响，筛选出含水率、含油率、Tween80为响应面的实验因素，对石油降解条件进行优化。结果表明：在选取的3个因素的水平范围内，对石油降解率的影响大小依次为：含油率含水率Tween80,含水率和含油率的交互作用对土壤中石油的降解率影响较显著。响应面模型优化后得到的最佳降解条件为土壤含水率58.2%、土壤含油率8.27%、Tween802.93CMC,此时土壤中石油的预期降解率能达到60.93%;验证实验得到的降解率为58.68%,达到理论预测值的97.73%。

关键词：白腐真菌；石油污染；降解；土壤

中图分类号：Q939.11文献标识码：A

Studyonremediationofpetroleumcontaminatedsoilbywhite-rotfungiTANLi-quan,YUMei,ZHOUTian°,PENGZhen-yu

(ColledgeofChemicalEngineering,GuangdongUniversityofPetrochemicalTechnology,Maoming525000,China)

Abstract:Crudeoilwaschoseasresponsesubstanceinordertostudythefactorsinfluenceoildegradationratebywhite-rotfungit.Thefactorsincludingthemoisturecontentofsoil,theoilcontentofsoil,therotationspeed,theconcentrationofTween80andtheconcentrationofveratrylalcohol.Themoisturecontent,oilcontent,Tween80inresponsetothetestwerefilteredoutasthefactorsbyresponsesurfacemethod.TheexperimentusedDesignEx-perttodotheresponsesurfaceoptimizationexperiment.Theresultsshowedthatwithinthescopeofthelevelsofthreefactors,theimpactontheoildegradationrateasfollows:oilcontentmoisturecontentTween80,moisturecontentandoilcontentofinteractionhasobviousimpactonoildegradationrateinthesoil.Thenthroughthere-sponsesurfacemethod,theoptimizedconditionsobtainedwere:moisturecontent57%,oilcontent8.42%and

Tween803.86CMC,thedegradationrateofpetroleumcanreachto60.93%.Theconfirmatoryexperimenthasfoundoutthattheactualdegradationrateofpetroleumis58.68%,whichhasreached97.73%