黄花补血草多糖的抗肿瘤作用研究.pdfVIP

黄花补血草多糖的抗肿瘤作用研究--第1页

黄花补血草多糖的抗肿瘤作用研究

龙玲;王军平;陈世恩;马殊雯;王明明;阿依木古丽;霍生东

【摘要】【Objective】Theresearchwastostudytheantitumoreffectsof

thestingsofpolysaccharidesfromLimoniumaureum(Linn.)(LAL-P)onmice

bearinghepatocellularcarcinoma(H22)andtheexpressionofBcl-2and

Bax.【Method】TheeffectsofLAL-Pontheinhibitionrateofsolidtumor

werestudiedbybuildingH22micetumormodel.Themorphologychanges

oftumorcellswereobservedbypathologicalsection.Theeffectsof

expressionchangesofpositivecellofBcl-2andBaxproteinofLAL-Pwere

observedbyimmunohistochemicalSPmethodtostudyitsantitumor

mechanism.【Result】Comparedwiththecontrolgroup,theLAL-P(120

mg/kgand240mg/kg)andCTX(25mg/kg)administrationssignificantly

reducedthetumorweightofsolidtumor.Theinhibitionratesofsolid

tumorwereupto31.32%,40.52%and56.61%respectively.Therewas

significantdifferencecomparedwiththecontrolgroup.Thetumorcellsof

LAL-P-treatedmiceshowedamassivenecrosissuchasnucleus

atrophy,disintegratingandstructurelessredstainingregion.Somecells

undergoingapoptosisshowedchromatincondensation,cell

shrinkage,plasmamembraneblebbingandtheformationofmembrane-

enclosedapoptoticbodies.Meanwhile,theproteinexpressionofBax

increasedandtheproteinexpressionofBcl-2decreasedafteroral

administrationofLAL-P.【Conclusion】LAL-Pshowedantitumoractivity

anditsmechanismmightberelatedwiththeup-regulationofBaxand

黄花补血草多糖的抗肿瘤作用研究--第1页

黄花补血草多糖的抗肿瘤作用研究--第2页

down-regulationofBcl-2proteinexpression.%【目的】研究黄花补血草多糖

对小鼠肝癌H22细胞生长的抑制作用及对Bcl-2和Bax蛋白表达的影响。【方法】

制备小鼠H22实体瘤模型。提取黄花补血草多糖,研究其对小鼠H22实体瘤的抑

瘤率,并通过病理切片观察肿瘤细胞的形态学变化。用免疫组化SP法检测黄花补血