响应曲面优化固定化微生物修复石油污染土壤.docx

石油学报(石油加工)

2015年8月ACTAPETROLEISINICA(PETROLEUMPROCESSINGSECTION)第31卷第4期

文章编号：1001-8719(2015)04-1028-07

响应曲面优化固定化微生物修复石油污染土壤

张涵，韩雨彤，张守娟，丁铮，张秀霞

(中国石油大学环境与安全工程系，山东青岛266580)

摘要：采用秸秆载体DG吸附高效石油降解菌SJ5-1制备固定化微生物。在花盆中模拟固定化微生物修复石油污染土壤，采用单因素实验考察wc:wn:wp(有机质、全氮、速效磷的质量分数的比值)、含水率、接种量对石油烃降解率的影响，再通过Design-Expert8.06响应曲面法设计实验，建立多元二次回归方程预测模型，进一步确定固定化微生物的最佳实验参数组合，并在最佳参数条件下验证模型的准确性。结果表明，预测模型准确；在土壤含油率为4.72%(质量分数)时，固定化微生物修复35d,响应面优化得到固定化微生物最佳降解条件为we:wN:w=

100:9.79:1,接种量10.34%、含水率17.8%(质量分数),石油烃降解率为42.4%,高于单因素最优条件时的40.8%;对石油烃降解率影响的显著性从大到小的因素依次是接种量、含水率、wc/wN。

关键词：固定化微生物；石油污染土壤；响应面；优化条件

中图分类号：X53文献标识码：Adoi:10.3969/j.issn.1001-8719.2015.04.029

OptimizationforPetroleum-ContaminatedSoilRemediatedbyImmobilizedMicroorganismsUsingResponseSurfaceMethod

ZHANGHan,HANYutong,ZHANGShoujuan,DINGZheng,ZHANGXiuxia

(DepartmentofEnvironmentalandSafetyEngineering,ChinaUniversityofPetroleum,Qingdao266580,China)

Abstract:StrawDGwasusedascarriermaterialforpetroleumhydrocarbondegradingstrainSJ5-1toprepareimmobilizedmicroorganisms.Inindoorflowerpot,theremediationofpetroleum-contaminatedsoilbyimmobilizedmicroorganismswassimulated,andtheimpactsofwc:WN:wp,moisturecontentandtheinoculumamountonhydrocarbondegradationratewereinvestigatedbysinglefactorexperiment.Thentheresponsesurfacemethodwasusedtodesigntheexperiment,bywhichapredictionmodelofquadraticpolynomialregressionequationwasobtainedtodeterminetheoptimalparameterarrangement.Undertheoptimizedparameterarrangement,themodelwasverified.Theresultsindicatedthatthemodelwasprovedveracious.Bycontrollingtheimmobilizedmicroorganismsinoculumbeing10.34%,wc/wn=10.21,moisturecontentbeing17.8%accordingtoresponsesurfaceanalysis,thebestdegradationrateof42.4%wasobtained,