Ento-Ia涂膜剂经皮给药对小鼠急性不完全性脑缺血的保护作用.pdfVIP

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Ento－Ia涂膜剂经皮给药对小鼠急性不完全性脑缺血的保护

作用

朱凤;胡园;刘衡;肖怀;杨自忠;赵海荣;张成桂;何苗

【摘要】ObjectiveToinvestigotetheprotectiveeffectofEnto-ⅠaPlaster

onacutemiceincompletecerebralischemia.Methods140micewere

dividedintotwobatchestocarryontheexperiment,eachbatch70.70rats

wererandomlydividedinto7groups,10ineachgroup,theshamoperation

group,theEnto-Ⅰacoatinggroup(37.50mg·kg-1、18.75mg·kg-1、9.

38mg·kg-1),thesalinemodelgroup,thematrixmodelgroup,the

nimodipinegroup(18mg·kg-1,ig).Ento-Iaplasticwasgiven

prophylacticallyfor5days,establishmentmiceacuteincompletecerebral

ischemiamodelbytotalbilateralcarotidarteryligationinmicewithvagus

nerve,recordsur-vivaltimeofmiceafterischemiaandthedeterminationof

mousebraintissueSODactivity,MDAcontentandthecontentofEvans

blue.ResultsThisresearchshowsthatComparedwiththemodelgroup

matrix,Ento-Iaplasticcansignificantlyprolongthesurvivaltimeofmice

afterligationofbilateralcommoncarotidarterywithvagusnerveatadose

of18.75mg·kg-1(P0.05),theextensionratewas39.55%;Atadoseof

137.50mg·kg-1,theeffectwasthebest(P0.01),prolongedsurvivaltime

ofmicewas45.15%,com-paredwiththepositivedrugnimodipine

(18mg·kg-1,theelongationrateof43.96%).Comparedwiththematrix

modelgroup,Ento-Iaplasticatthedoseof18.75and37.50mg·kg-1dose

groupcouldincreasethebraintissueSODactivityandreduceMDA

Ento-Ia涂膜剂经皮给药对小鼠急性不完全性脑缺血的保护作用--第1页

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content(middledosegroup,P0.05;highdosegroupP0.01).Atthe

sametime,Ento-Iaplasticcansignificantlyreducethe

cerebralnbsp;capillaryperm