多星韭花朵呈色基因AwDFRs和AwFLS1的功能研究.pdf

目录

摘要·············································································IV

ABSTRACT····································································VII

第一章文献综述································································1

1.1多星韭的研究现状·······················································1

1.2植物花色研究进展·······················································2

1.3花色苷的研究现状·······················································2

1.4花色呈色关键结构基因的研究进展··································3

1.4.1花色苷的结构性质··················································3

1.4.2花色苷的生物合成··················································4

1.4.3二氢黄酮醇4-还原酶的研究进展·······························7

1.4.4黄酮醇合酶的研究进展············································8

1.5本文的研究意义与内容·················································9

第二章多星韭DFR基因的克隆与功能解析····························10

2.1实验材料·································································10

2.1.1仪器设备····························································10

2.1.1植物材料····························································10

2.1.2溶液配制·····························································11

2.1.3菌种··································································13

2.2实验方法·································································14

2.2.1AwDFRs基因的克隆·············································14

2.2.2AwDFRs的生物信息学分析····································19

2.2.3AwDFRs的表达量分析··········································20

2.2.4AwDFRs原核表达载体的构建与体外酶活检测·············20

2.2.5AwDFRs真核表达载体的构建与功能鉴定··················24

2.3结果与分析······························································26

2.3.1AwDFRs基因的克隆·············································26

2.3.2AwDFRs的生物信息学分析····································26

2.3.3AwDFR