益骨汤对去势大鼠骨密度及经典BMP2信号通路的影响.docx

益骨汤对去势大鼠骨密度及经典BMP2信号通路的影响

林晓芳方芳李桂锦姚新苗

[摘要]?目的?觀察益骨汤对去势大鼠骨组织中经典骨形态发生蛋白（bone?morphogenetic?protein，BMP）2信号通路的影响，旨在探讨益骨汤防治骨质疏松症（osteoporosis，OP）的作用机制。方法?将44只12周龄雌性Wistar大鼠随机分为四组，每组11只。正常组不进行任何处理，模型组、戊酸雌二醇组和益骨汤组大鼠进行去势手术以模拟骨质疏松。造模成功后，分别以10ml/kg双蒸水灌胃（正常组、模型组）、0.36mg/kg戊酸雌二醇水溶液灌胃及10ml/kg益骨汤灌胃，??????????1次/d。12周后，双能X射线骨密度仪测定股骨骨密度（bone?mineral?density，BMD），实时聚合酶链反应（polymerase?chain?reaction，PCR）检测BMP2经典信号通路BMP2、Smad1、Runx2、OSX基因的表达，免疫组织化学法、Western?blot法检测BMP2经典信号通路BMP2、Smad1、Runx2、OSX蛋白的表达量。结果?①双能X射线骨密度仪测定股骨骨密度，结果显示，与模型组比较，益骨汤组大鼠的BMD升高（P0.01）。②实时荧光定量PCR检测，与模型组比较，益骨汤组大鼠的BMP2、Smad1、Runx2、Noggin基因表达量升高（P0.05）。③免疫组织化学法检测：与模型组比较，益骨汤组大鼠的BMP2、Smad1、Runx2、OSX蛋白表达量升高（P0.05）。④Western?blot法检测：与模型组比较，益骨汤组大鼠的Smad1、Runx2、OSX蛋白含量升高（P0.05）。结论?益骨汤可能通过激活BMP2经典信号通路及抑制Noggin的高表达，促进骨形成和防治骨质疏松。

[关键词]?益骨汤；去势大鼠；骨质疏松；BMP2经典信号通路；Noggin

[中图分类号]?R33??????[文献标识码]?A??????[DOI]?10.3969/j.issn.1673-9701.2024.06.017

Effect?of?Yigu?decoction?on?classical?BMP2?signaling?pathway?in?bone?tissue?of?ovariectomized?rats

LIN?Xiaofang1，?FANG?Fang2，?LI?Guijin3，?YAO?Xinmiao3

1.Department?of?Orthopedics，?Taizhou?Hospital?of?Zhejiang?Province，?Linhai?317000，?Zhejiang，?China;?2.Laboratory?of?Acupuncture?Neurobiology，?the?Third?Clinical?School?of?Medicine，?Zhejiang?Chinese?Medicine?University，?Hangzhou?310053，?Zhejiang，?China;?3.Department?of?Orthopaedics，?the?Third?Affiliated?Hospital?of?Zhejiang?Chinese?Medicine?University，?Hangzhou?310053，?Zhejiang，?China

[Abstract]?Objective?To?observe?the?impact?of?Yigu?decoction?on?the?classic?bone?morphogenetic?protein?（BMP）2?signaling?pathway?in?rat?bone?organization，?aim?to?explore?the?mechanism?of?Yigu?decoction?in?preventing?and?treating?osteoporosis?（OP）.?Methods?A?total?of?44?12-week-old?female?Wistar?rats?were?randomly?divided?into?four?groups，?with?11?rats?in?each?group.?The?normal?group?did?not?receive?any?treatment，?while?the?model?group，?estradiol?valerate?group?and?Yigu?decoction?group?were?castrated?to?simulate?osteoporosis.?After?successful?modeling，?the?rats?we